BIOMAT Database Logo BIOMAT Database Logo

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti. 1995

D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
Department of Biology, University of Victoria, B.C., Canada.

The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl amino-peptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.

UI MeSH Term Description Entries
D007987 Gonadotropin-Releasing Hormone A decapeptide that stimulates the synthesis and secretion of both pituitary gonadotropins, LUTEINIZING HORMONE and FOLLICLE STIMULATING HORMONE. GnRH is produced by neurons in the septum PREOPTIC AREA of the HYPOTHALAMUS and released into the pituitary portal blood, leading to stimulation of GONADOTROPHS in the ANTERIOR PITUITARY GLAND. FSH-Releasing Hormone,GnRH,Gonadoliberin,Gonadorelin,LH-FSH Releasing Hormone,LHRH,Luliberin,Luteinizing Hormone-Releasing Hormone,Cystorelin,Dirigestran,Factrel,Gn-RH,Gonadorelin Acetate,Gonadorelin Hydrochloride,Kryptocur,LFRH,LH-RH,LH-Releasing Hormone,LHFSH Releasing Hormone,LHFSHRH,FSH Releasing Hormone,Gonadotropin Releasing Hormone,LH FSH Releasing Hormone,LH Releasing Hormone,Luteinizing Hormone Releasing Hormone,Releasing Hormone, LHFSH
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D001923 Brain Chemistry Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states. Chemistry, Brain,Brain Chemistries,Chemistries, Brain
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005260 Female Females
D005399 Fishes A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013058 Mass Spectrometry An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass

Related Publications

D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
Characteristics of arylsulfatase in Russian sturgeon (Acipenser gueldenstaedti) semen.
December 2004, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
The complete mitochondrial genome of Russian sturgeon (Acipenser gueldenstaedti).
January 2016, Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis,
D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
Mammalian gonadotropin-releasing hormone (GnRH) receptor subtypes.
April 2002, Archives of physiology and biochemistry,
D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
Gonadotropin-Releasing Hormone (GnRH) Receptor Structure and GnRH Binding.
January 2017, Frontiers in endocrinology,
D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
Comparative distribution of mammalian GnRH (gonadotrophin-releasing hormone) and chicken GnRH-II in the brain of the immature Siberian sturgeon (Acipenser baeri).
November 1993, The Journal of comparative neurology,
D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
Isolation and characterization of [Pro2]somatostatin-14 and melanotropins from Russian sturgeon, Acipenser gueldenstaedti Brandt.
July 1995, General and comparative endocrinology,
D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
Gonadotropin-releasing hormone (GnRH): from fish to mammalian brains.
December 2002, Cellular and molecular neurobiology,
D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
[Gonadotropin releasing hormone (GnRH)].
March 1995, Nihon rinsho. Japanese journal of clinical medicine,
D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
[Gonadotropin releasing hormone (GnRH)].
December 1999, Nihon rinsho. Japanese journal of clinical medicine,
D W Lescheid, and J F Powell, and W H Fischer, and M Park, and A Craig, and O Bukovskaya, and I A Barannikova, and N M Sherwood
[Gonadotropin releasing hormone (GnRH)].
August 2005, Nihon rinsho. Japanese journal of clinical medicine,
Copied contents to your clipboard!