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The differentiation of HLA-B*1401 (B64) and B*1402 (B65) by the polymerase chain reaction using sequence-specific primers (PCR-SSP). 1995

M G Guttridge, and J Thompson, and C Burr, and C Darke
Regional Tissue Typing Laboratory, National Blood Transfusion Service Wales, Cardiff, United Kingdom.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D015235 HLA-B Antigens Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes. Antigens, HLA-B,HLA-B Antigen,HLA-B,Antigen, HLA-B,Antigens, HLA B,HLA B Antigen,HLA B Antigens
D015343 DNA Probes, HLA DNA probes specific for the human leukocyte antigen genes, which represent the major histocompatibility determinants in humans. The four known loci are designated as A, B, C, and D. Specific antigens are identified by a locus notation and number, e.g., HLA-A11. The inheritance of certain HLA alleles is associated with increased risk for certain diseases (e.g., insulin-dependent diabetes mellitus). DNA Probes, Histocompatibility Antigen,HLA DNA Probes,Histocompatibility Antigen DNA Probes,Human Leukocyte Antigen DNA Probes,HLA-A DNA Probes,HLA-B DNA Probes,HLA-C DNA Probes,HLA-D DNA Probes,HLA-DR DNA Probes,HLA-Dw DNA Probes,DNA Probes, HLA-A,DNA Probes, HLA-B,DNA Probes, HLA-C,DNA Probes, HLA-D,DNA Probes, HLA-DR,DNA Probes, HLA-Dw,HLA A DNA Probes,HLA B DNA Probes,HLA C DNA Probes,HLA D DNA Probes,HLA DR DNA Probes,HLA Dw DNA Probes,Probes, HLA DNA,Probes, HLA-A DNA,Probes, HLA-B DNA,Probes, HLA-C DNA,Probes, HLA-D DNA,Probes, HLA-DR DNA,Probes, HLA-Dw DNA
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D059909 HLA-B14 Antigen A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*14 allele family. HLA B14,HLA B14 Antigen,HLA Class I Histocompatibility Antigen, B-14 alpha Chain,HLA-B14,Antigen, HLA B14,Antigen, HLA-B14,B14 Antigen, HLA,HLA Class I Histocompatibility Antigen, B 14 alpha Chain

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