-transparent.png){kind=logo}
Vasoactive intestinal peptide (VIP) is a known pulmonary and bronchial vasodilator as well as an oxygen free radical scavenger. Since its effect as an additive to University of Wisconsin (UW) solution for lung preservation has been shown previously, the aim of this study was to determine the ability of VIP to improve lung preservation followed by reperfusion. Four groups of excised Sprague-Dawley rat lungs (n = 24) were studied using an isolated blood perfused working lung model. The first 3 groups of lungs were flushed and stored in UW solution at 4 degrees C for: (1) 4 hr, (2) 18 hr, and (3) 24 hr. Group 4 lungs were flushed with UW solution + VIP (1 microgram/ml) and stored in UW solution + VIP (0.5 microgram/ml) for 24 hr. After preservation, the lungs were reperfused to evaluate their functions for 2 hr or until lung failure occurred (arterial oxygen saturation less than 90% and/or appearance of bronchial fluid in the bronchial cannula). In the lungs stored in UW solution for 24 hr, failure occurred after 10 min of reperfusion and all functions were significantly altered. The addition of VIP to UW solution maintained the functional capacity of the lungs, recorded by lung resistance, lung compliance, elastic work, flow resistive work, shunt fraction, and blood oxygen tension. No statistical difference in these parameters other than shunt fraction was found when the VIP group was compared with the group preserved for 4 hr in UW solution. We conclude that lung preservation can be extended to 24 hr with the maintenance of lung functional capacity if VIP is added to UW solution.
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
February 1991,
Transplantation proceedings,
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
May 1993,
The Annals of thoracic surgery,
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
October 1993,
Transplantation,
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
June 1989,
Lancet (London, England),
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
April 1991,
The Journal of surgical research,
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
February 1993,
Transplantation proceedings,
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
October 1991,
Transplantation proceedings,
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
May 1994,
The Journal of thoracic and cardiovascular surgery,
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
October 1995,
Transplantation proceedings,
F Alessandrini, and
S Sasaki, and
S I Said, and
R Lodi, and
J LoCicero
November 1998,
Circulation,
