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A method of preparative isolation of membrane rIIB protein from bacteriophage T4 is worked out. Conditions are found to maximal rIIB protein accumulation in membranes of E. coli cells infected with bacteriophage T4. The membrane isolation by ultracentrifugation is substituted with their sedimentation from polyethyleneglycol solutions by low-speed centrifugation. A fraction, enriched with rIIB protein, is obtained using the treatment of cell walls with detergents. Preparative polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate was used for further rIIB protein purification. The method described can be applied for the purification and preparative isolation of memorane and poor-soluble proteins. The problem on location and ufunctioning of rIIB protein is discussed.
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
August 1970,
Virology,
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
February 1978,
Molecular & general genetics : MGG,
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
May 1977,
Virology,
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
July 1974,
Virology,
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
February 1994,
Doklady Akademii nauk,
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
March 1979,
Molecular & general genetics : MGG,
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
January 1964,
Biochemical and biophysical research communications,
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
April 1975,
Journal of virology,
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
July 2000,
Genetika,
N A Shevchenko, and
P Ia Boĭkov, and
M V Lichina, and
S D Kononova, and
L L Gumanov
January 1966,
Journal of bacteriology,
