Biomaterial Database

Kinetics of the repression of tylosin biosynthesis by ammonium ion in Streptomyces fradiae. 1994

S H Lee, and K J Lee

Department of Microbiology, College of Natural Sciences, Seoul National University, South Korea.

Nitrogen regulation of tylosin synthesis in Streptomyces fradiae NRRL 2702 was studied in batch and chemostat cultures using a soluble synthetic medium. In batch cultures, valine dehydrogenase (VDH; EC 1.4.1.8), threonine dehydratase (TDT; EC 4.2.1.16) and aspartate aminotransferase (ASAT; EC 2.6.1.1) reached their highest specific activities at 120 h. The specific activities of the three enzymes showed close correlation with the value of specific tylosin formation rate (qTYL). In chemostat cultures, the maximum value of qTYL was 1.14 tylosin per mycelial mass per h (mg g-1 h-1) at the specific growth rate of 0.05 h-1, and after reaching a rate of 0.1 h-1, qTYL decreased with increasing levels of the specific growth rate. This value of qTYL was 3.5-times as large as that of maximum qTYL observed in the batch culture. The specific formation rates of VDH, TDT, ASAT and tylosin were repressed by high levels of specific ammonium ion uptake rate.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D000644	Quaternary Ammonium Compounds	Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN	Quaternary Ammonium Compound,Ammonium Compound, Quaternary,Ammonium Compounds, Quaternary,Compound, Quaternary Ammonium
D001219	Aspartate Aminotransferases	Enzymes of the transferase class that catalyze the conversion of L-aspartate and 2-ketoglutarate to oxaloacetate and L-glutamate. EC 2.6.1.1.	Aspartate Aminotransferase,Aspartate Transaminase,Glutamic-Oxaloacetic Transaminase,SGOT,Aspartate Apoaminotransferase,Glutamate-Aspartate Transaminase,L-Aspartate-2-Oxoglutarate Aminotransferase,Serum Glutamic-Oxaloacetic Transaminase,Aminotransferase, Aspartate,Aminotransferase, L-Aspartate-2-Oxoglutarate,Aminotransferases, Aspartate,Apoaminotransferase, Aspartate,Glutamate Aspartate Transaminase,Glutamic Oxaloacetic Transaminase,Glutamic-Oxaloacetic Transaminase, Serum,L Aspartate 2 Oxoglutarate Aminotransferase,Serum Glutamic Oxaloacetic Transaminase,Transaminase, Aspartate,Transaminase, Glutamate-Aspartate,Transaminase, Glutamic-Oxaloacetic,Transaminase, Serum Glutamic-Oxaloacetic
D013302	Streptomyces	A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
D013913	Threonine Dehydratase	A pyridoxal-phosphate protein that catalyzes the deamination of THREONINE to 2-ketobutyrate and AMMONIA. The role of this enzyme can be biosynthetic or biodegradative. In the former role it supplies 2-ketobutyrate required for ISOLEUCINE biosynthesis, while in the latter it is only involved in the breakdown of threonine to supply energy. This enzyme was formerly listed as EC 4.2.1.16.	Threonine Deaminase,Threonine Dehydrase,Threonine Ammonia-Lyase,Ammonia-Lyase, Threonine,Deaminase, Threonine,Dehydrase, Threonine,Dehydratase, Threonine,Threonine Ammonia Lyase
D015645	Tylosin	Macrolide antibiotic obtained from cultures of Streptomyces fradiae. The drug is effective against many microorganisms in animals but not in humans.	Fradizine,Tylan,Tylosin Hydrochloride,Tylosin Tartrate,Tylosin Tartrate (Salt),Tylosine,Hydrochloride, Tylosin,Tartrate, Tylosin