-transparent.png){kind=logo}
An extracellular proteinase secreted by Candida pulcherrima KSY 188-5 was purified about 60-fold to electrophoretical homogeneity from its culture supernatant, by ammonium sulfate fractionation, anion-exchange chromatography, and gel filtration. The proteinase had a molecular weight of approximately 36,500 and an isoelectric point of pH 4.7. The enzyme had an optimum pH of around 2.5-3.5 for activity and 3.0-5.0 for stability. The optimum temperature was around 45 degrees C at pH 3.0. The enzyme showed a broad substrate specificity for a variety of proteins to hydrolyze casein, BSA, hemoglobin keratin, and collagen. Among several proteinase inhibitors, pepstatin A completely abolished the enzyme activity; indicating that the extracellular proteinase from C. pulcherrima KSY 188-5 was classified in the group of carboxyl proteinases.
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
June 1984,
The Journal of biological chemistry,
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
May 1986,
The Biochemical journal,
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
January 2001,
Infection and immunity,
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
January 1992,
Microbiology and immunology,
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
July 2012,
Medical mycology,
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
May 1974,
European journal of biochemistry,
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
January 1994,
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology,
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
March 1987,
Biochimica et biophysica acta,
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
February 1992,
Biochemical Society transactions,
T Gotoh, and
K Kikuchi, and
K Kodama, and
H Konno, and
T Kakuta, and
T Koizumi, and
K Nojiro
January 2020,
Methods in molecular biology (Clifton, N.J.),
