Biomaterial Database

Ontogeny of follicle-stimulating hormone receptor gene expression in the rat testis and ovary. 1995

A S Rannikki, and F P Zhang, and I T Huhtaniemi

Department of Physiology, University of Turku, Finland.

The ontogeny of the follicle-stimulating hormone (FSH) receptor (R) gene expression was studied in the rat testis and ovary between day 12.5 or 14.5 of fetal life (f), respectively, and adulthood. In Northern blots hydbridized with a cRNA probe corresponding to a part of the extracellular domain of the FSHR, specific hybridization to testicular RNA was detected from day f18.5, and to ovarian RNA from postnatal day 7 onwards. The main transcripts in the testis were at all ages 7.0 kb and 2.5 kb in size. In the ovary, the main transcript was always 2.5 kb in size. In order to increase the sensitivity of mRNA detection, the FSHR gene expression was also analyzed using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique with primer pairs corresponding to the near full-length FSHR mRNA or to its extracellular domain. The specificity of the PCR products was verified by Southern hybridization using a nested 32P-labeled cDNA probe. The results indicated that the expression of the extracellular domain of the FSHR was first detected on day f14.5 in the testis and on day f20.5 in the ovary. The full-length mRNA appeared in both sexes 2 days later, which is in agreement with earlier measurements of appearance of FSHR binding in the rat testis (day f17.5) and ovary (day 3 post partum). In situ hybridization using an antisense cRNA probe for FSHR demonstrated that, as early in development as specific hybridization was detected, it was confined to the Sertoli cells in the testis and to granulosa cells in the ovary. When compared with the developmental onset of the LHR gene expression (our earlier data), a major difference was observed in the ovary; the message encoding the extracellular LHR domain appeared > 10 days earlier than that corresponding to the full-length LHR message. In the case of mRNAs for the testicular LHR, and for FSHR of both sexes, the difference between the developmental appearance of the truncated and full-length RNA forms was only 2 days.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010053	Ovary	The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.	Ovaries
D011962	Receptors, FSH	Cell surface proteins that bind FOLLICLE STIMULATING HORMONE with high affinity and trigger intracellular changes influencing the behavior of cells.	FSH Receptors,Follicle-Stimulating Hormone Receptors,Receptors, Follicle-Stimulating Hormone,FSH Receptor,Follicle-Stimulating Hormone Receptor,Follicle Stimulating Hormone Receptor,Follicle Stimulating Hormone Receptors,Hormone Receptor, Follicle-Stimulating,Hormone Receptors, Follicle-Stimulating,Receptor, FSH,Receptor, Follicle-Stimulating Hormone,Receptors, Follicle Stimulating Hormone
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012333	RNA, Messenger	RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.	Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D013737	Testis	The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.	Testicles,Testes,Testicle
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain