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The tissue inhibitors of metalloproteinases (TIMPs) comprise a family of proteins, of which two members have so far been described in humans. We have cloned and sequenced a third human TIMP (hTIMP-3) from phorbol ester-differentiated THP-1 cells stimulated with bacterial lipopolysaccharide. The open reading frame encodes a 211-amino-acid precursor including a 23-residue secretion signal. The mature polypeptide has a calculated molecular weight of 21.6 kD and includes an N-linked glycosylation site near the carboxyl terminus. The protein is quite basic, having a predicted isoelectric point of 9.04. We have mapped the single gene encoding human TIMP-3 to chromosome 22. By Northern analysis, transcripts for TIMP-3 were identified in a broad cross-section of tissues examined from both embryonic and adult origin. In all tissues except the placenta, the predominant transcript was 5.0 kb in size, with minor bands around 2.4 and 2.6 kb comprising no more than about 10% of the signal. In the placenta, the smaller bands accounted for close to 50% of the signal. Human TIMP-3 shows slightly closer amino acid sequence similarity to TIMP-2 (44.3%) than to TIMP-1 (38.4%), but is most closely related to a recently reported chicken TIMP, chIMP-3 (80.8% amino acid; 77.7% nucleic acid similarity.
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
May 1996,
Gene,
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
October 1996,
The Journal of biological chemistry,
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
December 1995,
Genome research,
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
January 1994,
Genomics,
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
April 1998,
Gene,
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
January 2003,
Advances in experimental medicine and biology,
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
November 1996,
The Journal of biological chemistry,
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
April 1990,
Proceedings of the National Academy of Sciences of the United States of America,
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
January 2001,
Biochimica et biophysica acta,
C G Wilde, and
P R Hawkins, and
R T Coleman, and
W B Levine, and
A M Delegeane, and
P M Okamoto, and
L Y Ito, and
R W Scott, and
J J Seilhamer
January 1992,
Connective tissue research,
