Biomaterial Database

Study on nucleic acid synthesis in mouse retina and retinal pigment epithelium by radioautography. 1995

Y Kong, and T Nagata

Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.

The ocular tissues of ddY mice from embryonic 9 to postnatal day 14 were labelled in vitro with 3H-thymidine or 3H-uridine. After radioautography, the radioautographs were observed by light and electron microscopes. The labeling indices (L.I.) with 3H-thymidine or grain numbers with 3H-uridine were calculated and the area (microns 2) of cell organelles in both labelled and unlabelled retinal cell with 3H-thymidine were measured by image analysis. The L.I. of retinal cells were low in the beginning then increased and reached a peak at P3, while the L.I. of retinal pigment epithelium (RPE) increased and reached a peak at P7. The L.I. of both retinal and PE cells disappeared at P14. However, the L.I. of anterior region were more than those of equaterial and posterior regions in both retinal and PE cells. The results of the image analysis showed that the average area of cell organelles, both labelled and unlabelled retinal cells decreased from E9 to P14. The grain numbers of retina labelled with 3H-uridine increased until P1 then decreased, while the numbers of RPE increased and reached a peak at P3 whereafter they declined. No significant differences were detected among the three regions.

UI	MeSH Term	Description	Entries
D008817	Mice, Mutant Strains	Mice bearing mutant genes which are phenotypically expressed in the animals.	Mouse, Mutant Strain,Mutant Mouse Strain,Mutant Strain of Mouse,Mutant Strains of Mice,Mice Mutant Strain,Mice Mutant Strains,Mouse Mutant Strain,Mouse Mutant Strains,Mouse Strain, Mutant,Mouse Strains, Mutant,Mutant Mouse Strains,Mutant Strain Mouse,Mutant Strains Mice,Strain Mouse, Mutant,Strain, Mutant Mouse,Strains Mice, Mutant,Strains, Mutant Mouse
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D009696	Nucleic Acids	High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.	Nucleic Acid,Acid, Nucleic,Acids, Nucleic
D010857	Pigment Epithelium of Eye	The layer of pigment-containing epithelial cells in the RETINA; the CILIARY BODY; and the IRIS in the eye.	Eye Pigment Epithelium
D012160	Retina	The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.	Ora Serrata
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001345	Autoradiography	The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)	Radioautography
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus