-transparent.png){kind=logo}
Phospholipid vesicles (liposomes) as membrane models have been used to study the penetration properties of peptide nucleic acid (PNA), a new DNA analog in which the nucleobases are attached to a pseudo-peptide backbone. The liposomes were characterised by carboxyfluorescein efflux, light-scattering and cryogenic transmission electron microscopy. The liposome structure was found not to be affected by the incorporation of PNA or an oligonucleotide. Two 10-mer fluorescein-labelled PNAs were found to have low efflux rates (half-times of 5.5 and 11 days), comparable to a 10-mer oligonucleotide (half-time of 7 days). We conclude that passive diffusion of unmodified PNA is not an effective way of transport into biological cells.
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
January 2003,
Bioconjugate chemistry,
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
April 2010,
Analytical biochemistry,
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
June 1949,
Journal of cellular and comparative physiology,
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
August 1999,
Journal of biomolecular structure & dynamics,
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
December 2022,
Biopolymers,
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
January 2019,
Biochimica et biophysica acta. Biomembranes,
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
March 1999,
Analytical biochemistry,
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
April 1979,
Photochemistry and photobiology,
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
September 2009,
Bioconjugate chemistry,
P Wittung, and
J Kajanus, and
K Edwards, and
P Nielsen, and
B Nordén, and
B G Malmström
January 2003,
Nucleosides, nucleotides & nucleic acids,
