Biomaterial Database

Glycol methacrylate embedding of bone marrow cores. 1995

J S Maj

Department of Laboratory Diagnostic and Clinical Immunology, Medical Academy, Warszawa.

The objective of the present study was to assess the use of glycol methacrylate (GMA) as an embedding medium for bone marrow biopsies. Paraffin-embedded bone marrow trephines, which are most widely used for diagnostic purposes, need to be decalcified prior to sectioning. This process has a number of effects, and results in interference with the evaluation of the bone marrow. Differentiation of some cases of hematological malignancies may be difficult in decalcified, paraffin-embedded bone marrow biopsy specimens, because of the reported inability to obtain reproducible blast counts, and because a considerable percentage of cells cannot be easily recognized. On the other hand, GMA-embedding of marrow cores, which obviates the need for decalcification, shows structural organization of the marrow and gives excellent cellular details. The simplicity of GMA embedding, short processing time and reproducibility, makes this method highly suitable for routine work in the hematology laboratory.

UI	MeSH Term	Description	Entries
D007085	Ilium	The largest of three bones that make up each half of the pelvic girdle.	Auricular Surface of Ilium,Iliac Crest,Iliac Crest Bone,Iliac Fossa,Bones, Iliac Crest,Crest Bone, Iliac,Crest Bones, Iliac,Crest, Iliac,Crests, Iliac,Fossa, Iliac,Iliac Crest Bones,Iliac Crests,Iliums
D008689	Methacrylates	Acrylic acids or acrylates which are substituted in the C-2 position with a methyl group.	Methacrylate
D001853	Bone Marrow	The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.	Marrow,Red Marrow,Yellow Marrow,Marrow, Bone,Marrow, Red,Marrow, Yellow
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001706	Biopsy	Removal and pathologic examination of specimens from the living body.	Biopsies
D015203	Reproducibility of Results	The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.	Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016613	Plastic Embedding	The infiltrating of histological specimens with plastics, including acrylic resins, epoxy resins and polyethylene glycol, for support of the tissues in preparation for sectioning with a microtome.	Embedding, Plastic