Biomaterial Database

The developing rat lung fibroblast and alveolar type II cell actively recruit surfactant phospholipid substrate. 1995

J S Nunez, and J S Torday

Department of Pediatrics, University of Maryland Medical School, Baltimore, MD 21201, USA.

Timely, adequate production of pulmonary surfactant is critical for survival at birth. This process is dependent on both maternal nutrition and glucocorticoids. Fetal rat lung lipofibroblast triglyceride accumulation is saturable, temperature-dependent and stimulated by glucocorticoids and cyclic AMP in primary cell culture. Spontaneous triglyceride uptake by fibroblasts increases ninefold between 17 and 21 days (= term) gestation and is stimulated 30-80% by exogenous glucocorticoid treatment. Coculture of Type II cells with fibroblasts preloaded with 3H-trioleate and increasing amounts of triglyceride results in a concomitant increase in saturated phosphatidylcholine (SPC) synthesized by Type II cells. Dexamethasone stimulates mobilization of fibroblast triglyceride incorporation into Type II cell-SPC by 36-40%. The hormonally regulated uptake and mobilization of triglyceride suggest active recruitment of surfactant phospholipid substrate by both alveolar connective tissue and epithelial cells.

UI	MeSH Term	Description	Entries
D008168	Lung	Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.	Lungs
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D011650	Pulmonary Alveoli	Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place.	Alveoli, Pulmonary,Alveolus, Pulmonary,Pulmonary Alveolus
D011663	Pulmonary Surfactants	Substances and drugs that lower the SURFACE TENSION of the mucoid layer lining the PULMONARY ALVEOLI.	Surfactants, Pulmonary,Pulmonary Surfactant,Surfactant, Pulmonary
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003907	Dexamethasone	An anti-inflammatory 9-fluoro-glucocorticoid.	Hexadecadrol,Decaject,Decaject-L.A.,Decameth,Decaspray,Dexasone,Dexpak,Hexadrol,Maxidex,Methylfluorprednisolone,Millicorten,Oradexon,Decaject L.A.
D005314	Embryonic and Fetal Development	Morphological and physiological development of EMBRYOS or FETUSES.	Embryo and Fetal Development,Prenatal Programming,Programming, Prenatal
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014304	Triolein	(Z)-9-Octadecenoic acid 1,2,3-propanetriyl ester.	Glycerol Trioleate,Trielaidin,Trioleate-Glycerin,Trioleoylglycerol,Trioleyl Glycerol,Glycerol, Trioleyl,Trioleate Glycerin,Trioleate, Glycerol