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We have found [125I]glucagon-like peptide-1(7-36)-amide-specific binding activity in rat skeletal muscle plasma membranes, with an estimated M(r) of 63,000 by cross-linking and SDS-PAGE. The specific binding was time and membrane protein concentration dependent, and displaceable by unlabeled GLP-1(7-36)-amide with an ID50 of 3 x 10(-9) M of the peptide; GLP-1(1-36)-amide also competed, whereas glucagon and insulin did not. GLP-1(7-36)-amide did not modify the basal adenylate cyclase activity in skeletal muscle plasma membranes. These data, together with our previous finding of a potent glycogenic effect of GLP-1(7-36)-amide in rat soleus muscle, and also in isolated hepatocytes, which was not accompanied by a rise in the cell cyclic AMP content, lead use to believe that the insulin-like effects of this peptide on glucose metabolism in the muscle could be mediated by a type of receptor somehow different to that described for GLP-1 in pancreatic B cells, where GLP-1 action is mediated by the cyclic AMP-adenylate cyclase system.
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
March 2004,
The Journal of endocrinology,
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
July 1998,
Acta diabetologica,
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
February 2017,
Physiological reports,
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
January 2001,
Recent progress in hormone research,
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
December 2000,
Journal of molecular endocrinology,
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
December 1984,
Diabetologia,
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
January 2018,
The Journal of endocrinology,
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
June 2016,
Current opinion in lipidology,
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
June 2007,
MMW Fortschritte der Medizin,
E Delgado, and
M A Luque, and
A Alcántara, and
M A Trapote, and
F Clemente, and
C Galera, and
I Valverde, and
M L Villanueva-Peñacarrillo
December 1999,
Nihon rinsho. Japanese journal of clinical medicine,
