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Ribosomal 30S protein S1 causes disruption of the secondary structure of certain pyrimidine-containing polynucleotides. Helical poly(U), poly(C, U), and neutral and acidic poly(C) are stoichiometrically converted by S1 to structures indistinguishable from their partially or completely thermally denatured forms, as revealed by circular dichroism. Of the several double- and triple-stranded helical polynucleotides tested that contain one polypurine strand and at least one polypyrimidine strand, only the conformation of the DNA.RNA hybrid, poly(A)-poly(dT), is perturbed. In the presence of S1, this hybrid undergoes a transition to a new structure that has a circular dichroism spectrum unlike either the native or thermally denatured forms. Intercalated ethidium bromide is released from poly(A)-poly(dT) by S1, confirming the occurrence of a conformational rearrangement. The translation inhibitor, autintricarboxylic acid, completely inhibits the action of S1 on polypyrimidines, but has no effect on the conformational perturbation of poly(A(-poly(dT). The possible relation between these observations and the biological function of protein S1 is discussed.
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
November 1977,
Proceedings of the National Academy of Sciences of the United States of America,
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
June 1976,
Biochemical and biophysical research communications,
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
December 1984,
Biochemistry,
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
January 1983,
Progress in nucleic acid research and molecular biology,
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
November 2008,
RNA (New York, N.Y.),
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
December 1979,
Nucleic acids research,
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
February 1983,
FEBS letters,
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
September 2019,
European biophysics journal : EBJ,
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
October 1994,
Biochemistry and molecular biology international,
D G Bear, and
R Ng, and
D Van Derveer, and
N P Johnson, and
G Thomas, and
T Schleich, and
H F Noller
August 2006,
RNA (New York, N.Y.),
