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We demonstrate that the initiation of coliphage lambda DNA replication is dependent on the host initiator function dnaA, provided that the lambdoid prophage Rac is absent. Presence of Rac compensated the absence of dnaA function, causing initiation of replication. In dnaAts rac+ cells at 43 degrees, most of parental phage DNA molecules, after one round of theta replication, switched to a replication with features of the sigma mode and produced progeny at high yield. Initiation of replication of the lambda Pts1 mutant at 43 degrees was blocked by dnaA function; however, under dnaA-rac+ conditions all parental phage DNA molecules, after one round of theta replication, switched to the sigma mode and produced progeny at high yield. Taking into account our recent finding that transcriptional activation of ori lambda seems to be dnaA-regulated (to be published elsewhere), we suggest that the DnaA-lambda Pts1 incompatibility occurs at the insertion of the ori lambda-bound lambda O-lambda P-DnaB preprimosome between the complementary lambda DNA strands. The role of Rac and the mechanism of the switch from theta to sigma mode of lambda phage DNA replication are discussed.
G Wegrzyn, and
A Wegrzyn, and
I Konieczny, and
K Bielawski, and
G Konopa, and
M Obuchowski, and
D R Helinski, and
K Taylor
November 1987,
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G Wegrzyn, and
A Wegrzyn, and
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M Obuchowski, and
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K Taylor
April 1997,
Molecules and cells,
G Wegrzyn, and
A Wegrzyn, and
I Konieczny, and
K Bielawski, and
G Konopa, and
M Obuchowski, and
D R Helinski, and
K Taylor
January 2021,
Frontiers in microbiology,
G Wegrzyn, and
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K Bielawski, and
G Konopa, and
M Obuchowski, and
D R Helinski, and
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July 1998,
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G Wegrzyn, and
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Biochimica et biophysica acta. Gene regulatory mechanisms,
G Wegrzyn, and
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G Wegrzyn, and
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G Wegrzyn, and
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G Wegrzyn, and
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G Wegrzyn, and
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