To investigate a possible role of the central serotonergic system in the central control of pancreatic exocrine secretion, the median raphe nucleus of the anesthetized rat was electrically stimulated. The parameters of the biphasic square wave stimulus were 2 V, 2 ms, and 40 Hz. Electrical stimulation of the median raphe nucleus for 10 min resulted in large increases in pancreatic volume flow and protein output. The pancreatic responses were completely abolished by spinal cord transection at the level between C4 and C5. Cervical vagotomy or adrenalectomy failed to attenuate the pancreatic responses. The median raphe stimulation evoked a remarkable elevation of the blood pressure of the carotid artery as well as the plasma concentrations of noradrenaline and adrenaline. The responses were also completely inhibited by spinal cord transection but unaffected by cervical vagotomy. Adrenalectomy blocked the elevation of the plasma concentration of adrenaline only. Noradrenaline, given intravenously, stimulated the pancreatic volume flow and protein output. The actions of noradrenaline were antagonized by propranolol but not by phentolamine. It is, therefore, concluded from the present study that the median raphe nucleus may play a stimulatory role in pancreatic exocrine secretion of anesthetized rats and that the stimulatory role is mediated via the sympathetic nervous system acting on beta-adrenoceptors.