BIOMAT Database Logo BIOMAT Database Logo

Comparison of HLA-DRB1 typing by DNA-RFLP, PCR-SSO and PCR-SSP methods and their application in providing matched unrelated donors for bone marrow transplantation. 1995

F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
Anthony Nolan Research Centre, Royal Free Hospital, London, England.

The aim of the study was to devise a strategy for large batch analysis to determine HLA Class II alleles exhibited by candidate bone marrow transplant donors and prospective recipients using previously published DNA-based typing techniques. Special attention was directed towards the technical aspects of procedures, the level of typing resolution and the speed of data analysis. 200 blood samples from volunteer bone marrow transplant donors typed serologically for HLA-DR and DQ were further investigated using three DNA-based typing methods: (i) restriction fragment length polymorphism (RFLP) analysis, (ii) polymerase chain reaction (PCR) amplification and subsequent hybridisation with sequence specific oligonucleotide probes (PCR-SSO), and (iii) PCR amplification with sequence specific primers (PCR-SSP) to resolve the DRB1* specificity of each individual. In general, the HLA-DR results obtained using PCR-SSO and PCR-SSP correlated well with each other. However, discordant results were obtained between PCR and RFLP based typing in 21 cases, especially in relation to DRB3* alleles associated with the DRB1 gene. These differences were due to three problems pertaining to RFLP analysis: i) alleles with identical DRB, DQA and DQB fragment sizes, ii) reliance on DQA and DQB results to assign the DRB genotype, and iii) a "new polymorphism" of DR7, in a DR7 homozygous, exhibiting a fragment similar in size to DR8. Our findings suggested a strategy requiring PCR-SSO analysis for initial low resolution class II typing involving large numbers of samples, while the use of PCR-SSP is reserved for small numbers of samples, for urgent samples or for situations where higher resolution is required.(ABSTRACT TRUNCATED AT 250 WORDS)

UI MeSH Term Description Entries
D008403 Mass Screening Organized periodic procedures performed on large groups of people for the purpose of detecting disease. Screening,Mass Screenings,Screening, Mass,Screenings,Screenings, Mass
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009927 Tissue and Organ Procurement The administrative procedures involved with acquiring TISSUES or organs for TRANSPLANTATION through various programs, systems, or organizations. These procedures include obtaining consent from TISSUE DONORS and arranging for transportation of donated tissues and organs, after TISSUE HARVESTING, to HOSPITALS for processing and transplantation. Organ Procurement,Organ Procurement Systems,Organ Shortage,Tissue Procurement,Tissue Shortage,Donor Cards,Organ Donation,Required Organ Donation Request,Required Request,Tissue Donation,Donor Card,Organ Donations,Organ Procurement System,Organ Procurements,Required Requests,Shortage, Tissue,Tissue Donations,Tissue Procurements,Tissue Shortages
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D005069 Evaluation Studies as Topic Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D005802 Genes, MHC Class II Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and include H-2M, I-A, and I-E loci in mice. Class II Genes,Genes, Class II,Genes, HLA Class II,MHC Class II Genes,Class II Gene,Gene, Class II
D006650 Histocompatibility Testing Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed) Crossmatching, Tissue,HLA Typing,Tissue Typing,Crossmatchings, Tissue,HLA Typings,Histocompatibility Testings,Testing, Histocompatibility,Testings, Histocompatibility,Tissue Crossmatching,Tissue Crossmatchings,Tissue Typings,Typing, HLA,Typing, Tissue,Typings, HLA,Typings, Tissue
D006684 HLA-DR Antigens A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS. HLA-DR,Antigens, HLA-DR,HLA DR Antigens
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
HLA typing by SSO and SSP methods.
January 2012, Methods in molecular biology (Clifton, N.J.),
F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
Bone marrow transplantation utilizing HLA-matched unrelated marrow donors.
June 1990, Blood reviews,
F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
Selection of unrelated bone marrow donors by PCR-SSP typing and subsequent nonradioactive sequence-based typing for HLA DRB1/3/4/5, DQB1, and DPB1 alleles.
November 1994, Tissue antigens,
F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
In vitro methods and selection of HLA-matched unrelated donors for bone marrow transplantation.
February 1991, Transplantation proceedings,
F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
Bone marrow transplantation for leukemia utilizing HLA-matched unrelated donors.
January 1993, Bone marrow transplantation,
F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
PCR-RFLP: a new HLA-DNA typing method tested in bone marrow transplantation.
February 1991, Transplantation proceedings,
F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
HLA-DRB1 DNA sequencing based typing: an approach suitable for high throughput typing including unrelated bone marrow registry donors.
January 2001, Tissue antigens,
F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
A comparison of DNA-RFLP typing with serology and mixed lymphocyte reaction in the selection of matched unrelated bone marrow donors.
September 1989, Bone marrow transplantation,
F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
PCR-SSO typing for DR4-Dw subtypes: application to unrelated bone marrow transplant donor selection.
January 1991, European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics,
F Jordan, and A J McWhinnie, and S Turner, and N Gavira, and A A Calvert, and S A Cleaver, and R H Holman, and J M Goldman, and J A Madrigal
Bone marrow transplantation from HLA-matched unrelated donors as treatment for leukemia.
January 1993, Journal of hematotherapy,
Copied contents to your clipboard!