[Distribution of the isoenzymes of nucleases in group A Streptococci (author's transl)]. 1976

E Tiesler, and U Beck
Fach Hygiene and Mikrobiologie der Universität des Saarlandes in Homburg/Saar.

62 strains of Group A streptococci of different origin were cultured under comparable conditions. The supernatants of cultures of the same optical density were investigated in respect of their DNase activity. After electrophoretic separation in PAA gel, the distribution and activity of the four isoenzymes, B, D, A and C was determined. The total activity showed a near-random distribution, all patterns except B D A were found. DNase B was produced by all, DNase C by one half, DNase A by one third, and DNase D by one fifth of the strains. DNase B alone was found in three out of ten strains. The total DNase-activity and the distribution pattern in strains of the same M serotype but of different origin varied within a wide range. So a further biochemical differentiation of this strain seems to be possible.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D003851 Deoxyribonucleases Enzymes which catalyze the hydrolases of ester bonds within DNA. EC 3.1.-. DNAase,DNase,Deoxyribonuclease,Desoxyribonuclease,Desoxyribonucleases,Nucleases, DNA,Acid DNase,Alkaline DNase,DNA Nucleases,DNase, Acid,DNase, Alkaline
D012703 Serotyping Process of determining and distinguishing species of bacteria or viruses based on antigens they share. Serotypings
D013297 Streptococcus pyogenes A species of gram-positive, coccoid bacteria isolated from skin lesions, blood, inflammatory exudates, and the upper respiratory tract of humans. It is a group A hemolytic Streptococcus that can cause SCARLET FEVER and RHEUMATIC FEVER. Flesh-Eating Bacteria,Streptococcus Group A,Bacteria, Flesh-Eating

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