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The ability to produce nitric oxide (NO) of human monocytes macrophages is object of debate. While studying the regulation of tumor necrosis factor (TNF) synthesis induced by endotoxin (LPS) in a human cell line of monocyte origin (THP-1) and in human peripheral blood mononuclear cells (PBMC) we found an indirect evidence of such production. We showed that L-N-monomethyl-arginine (L-NMMA), an inhibitor of NO synthase, and hemoglobin, a chelator of NO, are able to significantly reduce TNF synthesis, indicating that NO production is induced by LPS and contributes to the induction of TNF. Since NO is a known cytostatic agent, we also studied the cytostatic effect of LPS, and demonstrated that it is reverted by L-NMMA. Although we were unable to show any nitrites/nitrates accumulation in the culture media, taken together our data give an indirect evidence of a physiologically relevant LPS-induced NO production in human monocytes-macrophages.
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
P Ghezzi, and
M Fratelli
April 1994,
Journal of immunology (Baltimore, Md. : 1950),
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
P Ghezzi, and
M Fratelli
March 1998,
Neuroreport,
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
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M Fratelli
February 2003,
Experimental and molecular pathology,
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
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M Fratelli
April 2021,
Scientific reports,
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
P Ghezzi, and
M Fratelli
February 2012,
Molecular biology reports,
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
P Ghezzi, and
M Fratelli
January 2008,
Prostaglandins, leukotrienes, and essential fatty acids,
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
P Ghezzi, and
M Fratelli
January 1999,
The American journal of physiology,
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
P Ghezzi, and
M Fratelli
December 2002,
Cellular immunology,
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
P Ghezzi, and
M Fratelli
December 2006,
Biochemical and biophysical research communications,
M Zinetti, and
G Fantuzzi, and
R Delgado, and
E Di Santo, and
P Ghezzi, and
M Fratelli
June 2008,
Innate immunity,
