Biomaterial Database

Tumor necrosis factor-alpha receptor expression on chondrocytes isolated from human articular cartilage. 1994

C I Westacott, and R M Atkins, and P A Dieppe, and C J Elson

Department of Pathology, University of Bristol, UK.

OBJECTIVE To determine whether the previously observed enhanced susceptibility of osteoarthritic (OA) cartilage to tumor necrosis factor-alpha (TNF-alpha) induced degradation could be due to differences in receptor expression. METHODS Using specific monoclonal antibodies flow cytometry was used to identify and quantify 2 TNF-alpha receptors on the surface of chondrocytes isolated from human articular cartilage. RESULTS The proportion of chondrocytes expressing p55 TNF-alpha receptor was significantly higher in populations from OA cartilage (p < 0.01) when compared with similar populations from non-arthritic cartilage. The number of p55 receptors/chondrocyte was also significantly higher (p < 0.01) in OA. A significant correlation (r = 0.72, p < 0.18) was found in OA between the numbers of chondrocytes expressing p75 receptor and the number of receptors/chondrocyte. CONCLUSIONS p55 TNF-alpha receptor expression is significantly increased on OA chondrocytes ex vivo. Enhanced expression of p55, purported to be the biologically active receptor, could contribute to OA cartilage degradation.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D010003	Osteoarthritis	A progressive, degenerative joint disease, the most common form of arthritis, especially in older persons. The disease is thought to result not from the aging process but from biochemical changes and biomechanical stresses affecting articular cartilage. In the foreign literature it is often called osteoarthrosis deformans.	Arthritis, Degenerative,Osteoarthrosis,Osteoarthrosis Deformans,Arthroses,Arthrosis,Arthritides, Degenerative,Degenerative Arthritides,Degenerative Arthritis,Osteoarthritides,Osteoarthroses
D002358	Cartilage, Articular	A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.	Articular Cartilage,Articular Cartilages,Cartilages, Articular
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D005260	Female		Females
D005434	Flow Cytometry	Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.	Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000368	Aged	A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available.	Elderly
D000369	Aged, 80 and over	Persons 80 years of age and older.	Oldest Old

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