Culekinin depolarizing peptide: a mosquito leucokinin-like peptide that influences insect Malpighian tubule ion transport. 1994

T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
Laboratory for Invertebrate Neuroendocrine Research, Texas A&M University, College Station 77843.

A peptide termed culekinin depolarizing peptide (CDP) was isolated from approximately 1.2 million mosquitos (94% Culex salinarius). The peptide was isolated on the basis of a rapid myotropic assay that utilized a hindgut preparation from Leucophaea maderae and a transepithelial voltage assay that used mosquito Malpighian tubules from Aedes aegypti. A 15% trifluoroacetic acid extraction from the mosquitos, two solid phase extraction steps, and six HPLC steps resulted in the isolation of 9.7 nmol of CDP. This value corresponds to approximately 8 fmol/mosquito. Edman degradation indicated the following sequence for CDP: Asn-Pro-Phe-His-Ser-Trp-Gly-NH2. The sequence was confirmed as the suspected C-terminal amide form of the peptide, since native and synthetic CDP had identical chemical and biological properties. CDP is a member of the leucokinin family of neuropeptides. The leucokinins have been found in three other insect species (Leucophaea maderae, Acheta domesticus and Locusta migratoria) where these peptides were isolated by their myotropic properties alone. CDP shares a C-terminal sequence homology (i.e., Phe-X-Ser-Trp-Gly-NH2) with the rest of the leucokinins. CDP corresponds to the strongest tubule depolarizing activity in the C. salinarius extract. These findings agree with previous structure-activity studies that suggest that mosquitos would contain a leucokinin-like factor that had Phe-His-Ser-Trp-Gly-NH2 as the C-terminal pentapeptide. This is the first leucokinin isolated from blood feeding or holometabolous insects.

UI MeSH Term Description Entries
D007422 Intestines The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE. Intestine
D008317 Malpighian Tubules Slender tubular or hairlike excretory structures found in insects. They emerge from the alimentary canal between the mesenteron (midgut) and the proctodeum (hindgut). Malpighian Tubule,Tubule, Malpighian,Tubules, Malpighian
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009119 Muscle Contraction A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments. Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009479 Neuropeptides Peptides released by NEURONS as intercellular messengers. Many neuropeptides are also hormones released by non-neuronal cells. Neuropeptide
D003058 Cockroaches Insects of the order Dictyoptera comprising several families including Blaberidae, BLATTELLIDAE, Blattidae (containing the American cockroach PERIPLANETA americana), Cryptocercidae, and Polyphagidae. Blaberidae,Blattaria,Blattidae,Blattodea,Cryptocercidae,Dictyoptera,Polyphagidae,Cockroach,Blattarias,Blattodeas,Cockroache,Cockroachs,Dictyopteras
D000330 Aedes A genus of mosquitoes (CULICIDAE) frequently found in tropical and subtropical regions. YELLOW FEVER and DENGUE are two of the diseases that can be transmitted by species of this genus. Aede
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017136 Ion Transport The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions. Antiport,Ion Cotransport,Ion Exchange, Intracellular,Symport,Uniport,Active Ion Transport,Facilitated Ion Transport,Passive Ion Transport,Cotransport, Ion,Exchange, Intracellular Ion,Intracellular Ion Exchange,Ion Transport, Active,Ion Transport, Facilitated,Ion Transport, Passive,Transport, Active Ion,Transport, Ion

Related Publications

T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
January 1996, The Journal of experimental biology,
T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
December 2000, Insect biochemistry and molecular biology,
T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
October 2021, Current opinion in insect science,
T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
January 2004, The Journal of experimental biology,
T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
October 2021, Current opinion in insect science,
T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
October 2021, Current opinion in insect science,
T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
November 1992, The Journal of experimental biology,
T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
July 1988, The Journal of experimental biology,
T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
January 2003, The Journal of experimental biology,
T K Hayes, and G M Holman, and T L Pannabecker, and M S Wright, and A A Strey, and R J Nachman, and D F Hoel, and J K Olson, and K W Beyenbach
June 1995, Regulatory peptides,
Copied contents to your clipboard!