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A reversed phase HPLC method was developed for the determination of synephrine in Zhishi Injection using methanol-water (1:1) containing sodium 1-pentane sulfonate (3.5%, G/V) and acetic acid (0.1%, V/V) as the mobile phase with UV detoction at 275nm. Linear response was obtained in the range of 8-64 micrograms/ml (r = 0.9998), the absolute recovery was 96.2-103.4%, and RSD 2.7%-6.0% (n = 7).
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