In spite of the clarification of some significant physiological factors of visual fatigue caused by VDT work, pupillary reflexes have not been studied as to how they are affected after prolonged visual work. This study examined visual function changes objectively in terms of pupillary reflexes and lens accommodative responses after a 4-hr VDT operation task. The relationship between the two functions was also examined. Two measurements in this paper revealed the physiological function changes due to VDT operation. The subjects involved were five students with an average age of 22.6 years. First, near-reflex measurement ascertained decreases in amplitude and the velocity of accommodation function after the visual task. Second, light-reflex measurement revealed a delay of the reflex, an increase in the amplitude of the reflex, and a decrease in pupil size after the visual task. A weak correlation between the decrease in pupil size and accommodation function was found. The occurrence of visual fatigue due to 4-hr VDT operation was also confirmed by CFF measurements and reported subjective visual symptoms in this experiment.