Biomaterial Database

New techniques for rearing black flies from pupae (Diptera: Simuliidae). 1994

F F Hunter, and S G Burgin, and D M Wood

Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada.

Simple techniques are described for collecting black fly pupae from streams using plastic strips and for rearing large numbers of adult black flies in inexpensive enclosures made of chicken wire and cloth mesh netting, and 2 methods are described for rearing adult black flies individually from pupae. The first method of rearing individual black flies uses 1.5-ml microcentrifuge tubules and the second uses easy-to-construct rearing chambers that provide moisture for the developing pupa and water for the adult to imbibe. Instructions for assembly are provided. Specimens obtained using these rearing chambers are of museum quality.

UI	MeSH Term	Description	Entries
D011679	Pupa	An inactive stage between the larval and adult stages in the life cycle of INSECTA.	Chrysalis,Pupae
D004772	Entomology	A discipline or occupation concerned with the study of INSECTS, including the biology and the control of insects.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012843	Simuliidae	A family of insects in the order DIPTERA, which include black flies, buffalo gnats and simulies. Several species are intermediate hosts (vectors) for the parasitic disease ONCHOCERCIASIS.	Black Flies,Simulium,Blackflies,Black Fly,Blackfly,Flies, Black,Fly, Black,Simuliums
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species