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An immunocytochemical analysis of hormonal status of transgenic rats containing human growth hormone gene has been done. The enhanced expression of the endogenous growth hormone gene was demonstrated with poly- and monoclonal antibodies inside somatatropes of pituitary. No activity of the heterologous growth hormone gene was revealed in kidney, pancreas or liver as it might be expected according to specificity of MT1 and TAT promotors. Transgenic animals of F0, F1 and F2 generation exhibited disturbance of functional morphology of glucagon and insulin producing cells. Lymphocyte infiltration was found in pancreatic islets. The transgenic rabbits and swine with the gene of releasing factor of human growth hormone did not reveal any severe disturbance. Although one swine demonstrated alterations in glucagon producing cells and one rabbit revealed a disturbed morphology of the stomach tissues. The data are discussed in relation to general problems of transgene activity and interaction with endogenous homolog.
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
January 1989,
Doklady Akademii nauk SSSR,
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
November 1989,
Endocrinology,
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
August 1989,
Endocrinology,
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
July 1988,
Molecular endocrinology (Baltimore, Md.),
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
January 1985,
Oxford surveys on eukaryotic genes,
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
January 1991,
Problemy endokrinologii,
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
August 1996,
The Journal of biological chemistry,
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
July 1993,
Jikken dobutsu. Experimental animals,
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
January 1986,
Cell and tissue research,
V K Kazakov, and
S J Rosochacki, and
T V Ignat'eva, and
G F Golinskiĭ, and
A F Smirnov
January 1990,
Endocrinology,
