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Transforming growth factor-beta 1 inhibited proliferation of a human ovarian carcinoma cell line (NIH-OVCAR-3). The inhibition of NIH-OVCAR-3 cell proliferation was accompanied by a decrease in clonogenic potential, evidenced by the reduced ability of TGF-beta 1-treated NIH-OVCAR-3 cells to form colonies on a plastic substratum. This rapid decrease of clonogenic potential, which was detected 6 h after addition of TGF-beta 1 was dose-dependent (IC50 = 4 pM). Fluorescence microscopy of DAPI-stained cells supported by electron-microscopic examination showed that TGF-beta 1 induced chromatin condensation and nuclear fragmentation. In addition, oligonucleosomal-sized fragments were detected in the TGF-beta 1-treated cells. These features indicated that TGF-beta 1 induced NIH-OVCAR-3 cell death by an apoptosis-like mechanism. This TGF-beta 1 apoptotic effect was subject to modulation by cell density. It was observed that an increase in cell density (up to 20 x 10(3) cells/cm2) protected NIH-OVCAR-3 cells against apoptosis induced by TGF-beta 1. Conditioned medium from high-density cultures of NIH-OVCAR-3 cells did not inhibit apoptosis induced by TGF-beta 1 on NIH-OVCAR-3 cells cultured at low density, suggesting that the protective effect of cell density was not related to the cell secretion of a soluble survival factor.
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
July 1997,
Zhonghua fu chan ke za zhi,
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
November 1996,
Journal of experimental therapeutics & oncology,
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
November 1992,
International journal of cancer,
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
January 1988,
Growth factors (Chur, Switzerland),
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
January 1992,
Cancer research,
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
January 1997,
Virchows Archiv : an international journal of pathology,
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
March 1993,
British journal of cancer,
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
June 1998,
Chinese medical journal,
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
May 1987,
Biochemical and biophysical research communications,
C Mathieu, and
S Jozan, and
P Mazars, and
M G Côme, and
A Moisand, and
A Valette
December 1988,
Cancer research,
