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The antitumour antibiotic actinomycin D normally binds to DNA by intercalation at sequences containing the CpG step, but in the presence of daunomycin it has been reported to interact with poly(dA-dT). This observation has neither been confirmed nor explained. Here we have used a photoreactive 7-azido derivative of actinomycin to study the effect of daunomycin on its binding to three DNA fragments. Daunomycin did indeed alter the binding of actinomycin to the DNA, such that the antibiotic was displaced from its primary GpC sites onto secondary sites in the DNA, though not to AT regions especially. These findings suggest a possible scientific explanation for the increased toxicity seen during combination chemotherapy with these two drugs.
G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
January 1991,
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G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
July 1989,
FEBS letters,
G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
September 1966,
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G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
May 1988,
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G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
July 1988,
Drug design and delivery,
G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
January 1988,
FEBS letters,
G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
July 2013,
Chemistry (Weinheim an der Bergstrasse, Germany),
G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
December 1987,
Biochemistry,
G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
February 1993,
Current opinion in biotechnology,
G S Ridge, and
C Bailly, and
D E Graves, and
M J Waring
February 1990,
Anti-cancer drug design,
