Biomaterial Database

[Beta-lactamase-producing Haemophilus influenzae isolated in Portugal, 1989-1992]. 1994

M P Bajanca Lavado, and I Casin, and M V Vaz Pato

Secteur Résistance Antibiotiques, INSA, Lisboa, Portugal.

Within the framework of a national multicentric study between 1989 and 1992, 118 strains of betalactamase producing Haemophilus influenzae were isolated. Biotyping demonstrated the predominance of biotypes I, II and III, with 22, 36 and 24% of the strains, respectively. Encapsulated strains accounted for 13% of the total; all, but one, were serotype b. The antimicrobial susceptibility test (dilution method) of the 118 ampicillin--resistant strains showed: 33.9% resistance to tetracycline, 29.7% to chloramphenicol, 10.2% to erythromycin, 9.3% to trimethoprim, 0.8% to rifampicin, and 29.7% of multiresistance. All strains were susceptible to augmentin, cefotaxime, ceftriaxone and ceprofloxacin. Ninety strains were screened for resistant plasmids. A large plasmid (30-50 Mdal) was isolated in 38.9% of the strains and a small plasmid (3-4.4 Mdal) in 10%. No plasmid was found in 51% of the strains. Isoelectric focusing of 54 beta-lactamases showed that all were type TEM-1 (pI = 5.4), with the exception of one, which was type TEM-2 (pI = 5.6).

UI	MeSH Term	Description	Entries
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D011174	Portugal	A country in southwestern Europe, bordering the North Atlantic Ocean, west of Spain. The capital is Lisbon.	Madeira Island,Portuguese Republic
D002701	Chloramphenicol	An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)	Cloranfenicol,Kloramfenikol,Levomycetin,Amphenicol,Amphenicols,Chlornitromycin,Chlorocid,Chloromycetin,Detreomycin,Ophthochlor,Syntomycin
D004352	Drug Resistance, Microbial	The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).	Antibiotic Resistance,Antibiotic Resistance, Microbial,Antimicrobial Resistance, Drug,Antimicrobial Drug Resistance,Antimicrobial Drug Resistances,Antimicrobial Resistances, Drug,Drug Antimicrobial Resistance,Drug Antimicrobial Resistances,Drug Resistances, Microbial,Resistance, Antibiotic,Resistance, Drug Antimicrobial,Resistances, Drug Antimicrobial
D004917	Erythromycin	A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.	Erycette,Erymax,Erythromycin A,Erythromycin C,Erythromycin Lactate,Erythromycin Phosphate,Ilotycin,T-Stat,Lactate, Erythromycin,Phosphate, Erythromycin,T Stat,TStat
D006193	Haemophilus influenzae	A species of HAEMOPHILUS found on the mucous membranes of humans and a variety of animals. The species is further divided into biotypes I through VIII.	Bacterium influenzae,Coccobacillus pfeifferi,Haemophilus meningitidis,Hemophilus influenzae,Influenza-bacillus,Mycobacterium influenzae
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000667	Ampicillin	Semi-synthetic derivative of penicillin that functions as an orally active broad-spectrum antibiotic.	Penicillin, Aminobenzyl,Amcill,Aminobenzylpenicillin,Ampicillin Sodium,Ampicillin Trihydrate,Antibiotic KS-R1,Omnipen,Pentrexyl,Polycillin,Ukapen,Aminobenzyl Penicillin,Antibiotic KS R1,KS-R1, Antibiotic,Sodium, Ampicillin,Trihydrate, Ampicillin
D001618	beta-Lactamases	Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins.	beta-Lactamase,beta Lactamase,beta Lactamases
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings