-transparent.png){kind=logo}
The affinity of rifampin to bond to Dacron permits implantation of a vascular prosthesis with anti-staphylococcal bioactivity. The performance of rifampin-bonded Dacron grafts was evaluated in vitro and in vivo to develop a bonding technique with optimal graft bioactivity which was then used for in situ reconstruction of a biofilm graft infection. In vitro bioactivity was measured at 24-hr intervals for three types of Dacron prostheses (plain, gelatin, and collagen-impregnated) exposed to rifampin at varied concentrations and immersion times. Gelatin-impregnated grafts demonstrated superior bioactivity (P < 0.05). Rifampin concentration and graft type had a greater effect on bioactivity than immersion time (P < 0.01). Gelatin-impregnated grafts immersed in a 60 mg/ml rifampin solution for 15 min produced optimum bioactivity. Six grafts prepared in this fashion were used to replace the canine aorta. The level and duration of in vivo antistaphylococcal activity to Staphylococcus aureus and Staphylococcus epidermidis were less (P < 0.05) than those measured in vitro, but rifampin levels exceeded the study strain maximum inhibitory concentration for up to 48 hr. In a canine model, the rifampin-bonded gelatin-impregnated (N = 14) or nonbonded control (N = 10) grafts were used as in situ replacement for an established aortic graft infection caused by S. epidermidis. Replacement with a rifampin-bonded graft resulted in successful anatomic healing of perigraft and anastomotic tissue. Persistent biofilm colonization was confirmed in 8 of 10 controls versus 4 of 14 rifampin-bonded grafts (P < 0.02).(ABSTRACT TRUNCATED AT 250 WORDS)
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
September 1996,
Journal of vascular surgery,
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
May 1994,
Annals of vascular surgery,
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
April 1994,
Journal of vascular surgery,
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
June 1991,
Journal of vascular surgery,
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
August 1993,
Cardiovascular surgery (London, England),
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
April 1997,
[Zasshi] [Journal]. Nihon Kyobu Geka Gakkai,
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
January 1999,
Surgery today,
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
August 1992,
Artificial organs,
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
April 1994,
Journal of vascular surgery,
V Gahtan, and
G E Esses, and
D F Bandyk, and
R T Nelson, and
E Dupont, and
J L Mills
November 1994,
Journal of vascular surgery,
