Small angle neutron scattering studies of structural characteristics of agarose gels. 1994

S Krueger, and A P Andrews, and R Nossal
Reactor Radiation Division, National Institute of Standards and Technology, Gaithersburg, MD 20899.

The 30 m small angle neutron scattering facility at the National Institutes of Standards and Technology has been used to examine neutron scattering from agarose gels formed in D2O. Differential scattering cross sections have been acquired over a continuous range of Q between 0.005 and 0.3 A-1. Subtle changes in gel structure are observed when pre-gelation agarose concentration is varied. Similarly, except when the gelling solution is rapidly cooled to a low temperature, the rate at which the gels are formed does not seem to have much effect. Clearer evidence of structural rearrangement is observed when the solvent quality is changed by the addition of dimethyl sulfoxide, or when the temperature of the gel is elevated above 70 degrees C. These data are consistent with a description of a randomly structured polymer network containing discrete self-similar, hydrogen-bonded, junctions normally of minimal thickness approximately 35-40 A.

UI MeSH Term Description Entries
D009502 Neutrons Electrically neutral elementary particles found in all atomic nuclei except light hydrogen; the mass is equal to that of the proton and electron combined and they are unstable when isolated from the nucleus, undergoing beta decay. Slow, thermal, epithermal, and fast neutrons refer to the energy levels with which the neutrons are ejected from heavier nuclei during their decay. Neutron
D002236 Carbohydrate Conformation The characteristic 3-dimensional shape of a carbohydrate. Carbohydrate Linkage,Carbohydrate Conformations,Carbohydrate Linkages,Conformation, Carbohydrate,Conformations, Carbohydrate,Linkage, Carbohydrate,Linkages, Carbohydrate
D005782 Gels Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
D012542 Scattering, Radiation The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Radiation Scattering,Radiation Scatterings,Scatterings, Radiation
D012685 Sepharose Agarose,Sepharose 4B,Sepharose C1 4B,4B, Sepharose C1,C1 4B, Sepharose
D012997 Solvents Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed) Solvent

Related Publications

S Krueger, and A P Andrews, and R Nossal
November 2014, Langmuir : the ACS journal of surfaces and colloids,
S Krueger, and A P Andrews, and R Nossal
August 1994, Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics,
S Krueger, and A P Andrews, and R Nossal
February 2000, Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics,
S Krueger, and A P Andrews, and R Nossal
January 1983, Annual review of biophysics and bioengineering,
S Krueger, and A P Andrews, and R Nossal
October 2012, Biomacromolecules,
S Krueger, and A P Andrews, and R Nossal
April 2010, Langmuir : the ACS journal of surfaces and colloids,
S Krueger, and A P Andrews, and R Nossal
August 2008, Langmuir : the ACS journal of surfaces and colloids,
S Krueger, and A P Andrews, and R Nossal
August 1994, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
Copied contents to your clipboard!