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Staphylokinase (SAK) expresses plasminogen activator activity by forming a complex with plasminogen. In order to elucidate the mechanism for the expression of enzymatic activity of the complex, a cross-linked staphylokinase/plasminogen (SAK/plg) complex was produced with disuccinimidyl suberate, and its enzymatic characteristics were compared with those of a streptokinase/plasminogen (SK/plg) complex. SAK/plg complex and SK/plg complex showed a band with a molecular weight of 110 kDa and 140 kDa by SDS-PAGE under non-reduced condition, respectively. Both complexes exhibited plasminogen activator activity in a concentration-dependent manner on fibrin film and synthetic chromogenic substrate assay. The kinetic analysis of enzymatic activity of both complexes was performed. The plasminogen activator activity of SAK/plg complex was enhanced about 5-fold in the presence of FCB-2. However, SK/plg complex showed only 1.7-fold increase in the presence of FCB-2. These findings indicate that the SAK/plg complex reacts with fibrin, and efficient plasminogen activation is induced on fibrin surface.
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
April 1994,
Journal of biochemistry,
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
June 1991,
The Journal of biological chemistry,
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
February 1960,
Nature,
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
October 1994,
Thrombosis research,
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
January 1975,
Acta biochimica Polonica,
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
October 2000,
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis,
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
January 1993,
Methods in enzymology,
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
March 2005,
FEBS letters,
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
January 1987,
Haemostasis,
K Okada, and
H Yuasa, and
Y Hagiya, and
H Fukao, and
S Ueshima, and
O Matsuo
August 1993,
Blood,
