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Monoclonal antibodies directed against proteins modified with the major membrane lipid peroxidation product, 4-hydroxy-2-nonenal, have been established and characterized. The monoclonal antibodies specific for HNE-modified proteins were raised by immunizing mice with a HNE-keyhole limpet hemocyanin conjugate. The resulting five monoclonal antibodies (mAbs HNEJ-1-5) recognized HNE-modified bovine serum albumin (BSA), but not native BSA in Western blot studies. Of the five mAbs, HNEJ-2 exhibited the highest affinity for HNE-modified proteins and a much higher affinity for the HNE-histidine adduct than the HNE-lysine or HNE-cysteine adducts. mAb HNEJ-2 did not cross-react with proteins that had been treated with other aldehydes, such as 1-hexenal, 2-hexenal, 4-hydroxy-2-hexenal, 2-nonenal, formaldehyde, or glutaraldehyde. These results suggest that the major epitope recognized by mAb HNEJ-2 is the Michael addition-type HNE-histidine adduct.
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
January 2002,
Methods in molecular biology (Clifton, N.J.),
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
July 1995,
Free radical biology & medicine,
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
July 1997,
Lipids,
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
October 2004,
Organic letters,
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
July 2004,
Free radical biology & medicine,
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
April 2020,
Data in brief,
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
February 2003,
The Journal of biological chemistry,
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
December 1995,
Archives of biochemistry and biophysics,
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
September 2009,
Chemical research in toxicology,
S Toyokuni, and
N Miyake, and
H Hiai, and
M Hagiwara, and
S Kawakishi, and
T Osawa, and
K Uchida
January 1988,
Cancer research,
