-transparent.png){kind=logo}
Lymphocytes secreting anti-IgC antibodies, rheumatoid factors (RF), can be detected in the peripheral bloods, synovial fluids, and bone marrows of patients with seropositive rheumatoid arthritis by using a direct plaque-forming cell (PFC) assay with sheep erythrocytes sensitized with reduced and alkylated rabbit IgG hemolysin. The autospecific nature of the RF produced by RF-PFC was indicated by inhibition studies in which the order of patency was human IgG greater than rabbit IgG greater than bovine IgG. In metabolic studies puromycin, cycloheximide, and venblastine suppressed RF-PFC. Cyclic AMP and cyclic GMP were without effect. A need was recognized for using full tissue culture media during the cell separation and plaquing procedures to optimize detection of the RF-PFC. RF-PFC may appear in the blood of patients intermittently despite their continuing presence in the bone marrow. They have been found in the peripheral blood, especially during acutely exacerbating polyarticular synovitis, generalized vasculities, or generally active, aggressive disease. RF-PFC were found in synovial effusions of new or recrduescent acute synovitis. RF-PFC were observed to disappear from the peripheral circulation and the bone marrow during therapy with cytotoxic drugs. The data are consistent with the hypothesis that the appearance of RF-PFC in the peripheral blood represents an anamnestic response to transiently appearing antigen. The nature of the antigen is not specified. The bone marrow may be a site of origin of RF-PFC.
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
September 1987,
Journal of immunological methods,
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
December 1968,
Journal of immunology (Baltimore, Md. : 1950),
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
January 1974,
Intervirology,
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
June 1991,
Pathology, research and practice,
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
March 1968,
Immunochemistry,
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
October 1967,
The Journal of allergy,
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
October 1979,
The Journal of experimental medicine,
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
February 1968,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
January 1984,
Acta haematologica,
J H Vaughan, and
T Chihara, and
T L Moore, and
D L Robbins, and
K Tanimoto, and
J S Johnson, and
R McMillan
June 1979,
Annals of the rheumatic diseases,
