Effects of benzo(a)pyrene and tetrachlorodibenzo(p)dioxin on fetal dolphin kidney cells: inhibition of proliferation and initiation of DNA damage. 1995

M J Carvan, and L P Flood, and B D Campbell, and D L Busbee
Department of Anatomy and Public Health, College of Veterinary Medicine, Texas A & M University, College Station 77843.

Dolphin kidney cells (CDK) were exposed in vitro to benzo(a)pyrene (BaP) in the presence or absence of 2,3,7,8-tetrachlorodibenzo(p)dioxin (TCDD), a cytochrome P450-inducing agent, and/or alpha-naphthoflavone (alpha NF), an inhibitor of cytochrome P450 induction. BaP inhibited mitosis in CDK cells in a dose-dependent manner. TCDD, while inhibiting cell proliferation, did not show a strict dose-dependent mode of action. BaP inhibition of mitosis was decreased by alpha NF, which also decreased the inhibitory effects of TCDD on CDK proliferation. BaP treatment initiated both 3H-thymidine incorporation and the increased alkali lability of DNA functions of the initiation of excision repair. Cells pre-treated with TCDD and then exposed to BaP exhibited increased BaP-DNA adduct levels and increased DNA excision repair. These data indicate that dolphin cells metabolized BaP in vitro as a function of cytochrome P450-associated activities, that BaP metabolites covalently bound to cellular DNA and initiated excision repair. Inhibition of the cytochrome P450-mediated metabolism of BaP decreased the BaP-associated inhibition of mitosis in dolphin cells.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004249 DNA Damage Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS. DNA Injury,DNA Lesion,DNA Lesions,Genotoxic Stress,Stress, Genotoxic,Injury, DNA,DNA Injuries
D004260 DNA Repair The removal of DNA LESIONS and/or restoration of intact DNA strands without BASE PAIR MISMATCHES, intrastrand or interstrand crosslinks, or discontinuities in the DNA sugar-phosphate backbones. DNA Damage Response
D004289 Dolphins Mammals of the families Delphinidae (ocean dolphins), Iniidae, Lipotidae, Pontoporiidae, and Platanistidae (all river dolphins). Among the most well-known species are the BOTTLE-NOSED DOLPHIN and the KILLER WHALE (a dolphin). The common name dolphin is applied to small cetaceans having a beaklike snout and a slender, streamlined body, whereas PORPOISES are small cetaceans with a blunt snout and rather stocky body. (From Walker's Mammals of the World, 5th ed, pp978-9) Amazon Dolphins,Baiji,Cephalorhynchus,Chinese River Dolphin,Delphinidae,False Killer Whale,Franciscana,Fraser's Dolphin,Ganges Dolphin,Gray Grampus,Humpback Dolphins,Irrawaddy River Dolphin,La Plata Dolphin,Lagenorhynchus,Lissodelphis,Many-Toothed Blackfish,Marine Dolphins,Melon-Headed Whale,Ocean Dolphins,Piebald Dolphins,Platanista,Pontoporia blainvillei,Pygmy Killer Whale,Right Whale Dolphins,Risso's Dolphin,Rough-Toothed Dolphin,Sousa,Susus,Tucuxi Dolphin,Whale, False Killer,Whale, Melon-Headed,Whale, Pygmy Killer,White-Beaked Dolphins,Amazon River Dolphins,Atlantic White-Sided Dolphins,Feresa attenuata,Ganges River Dolphin,Grampus griseus,Inia geoffrensis,Lagenodelphis hosei,Lagenorhynchus acutus,Lagenorhynchus albirostris,Langenorhynchus obliquidens,Lipotes vexillifer,Orcaella brevirostris,Pacific White-Sided Dolphins,Peponocephala electra,Planista gangetica,Pseudorca crassidens,Sotalia fluviatilis,Steno bredanensis,Yangtze River Dolphin,Atlantic White Sided Dolphins,Atlantic White-Sided Dolphin,Baijus,Dolphin,Dolphin, Fraser's,False Killer Whales,Franciscanas,Fraser Dolphin,Frasers Dolphin,Humpback Dolphin,Lagenodelphis hoseus,Lagenorhynchus albirostri,Langenorhynchus obliquiden,Lissodelphi,Many Toothed Blackfish,Marine Dolphin,Melon Headed Whale,Melon-Headed Whales,Ocean Dolphin,Orcaella brevirostri,Pacific White Sided Dolphins,Pacific White-Sided Dolphin,Piebald Dolphin,Planista gangeticas,Platanistas,Pontoporia blainvilleus,Pseudorca crassiden,Pygmy Killer Whales,Right Whale Dolphin,Risso Dolphin,Rissos Dolphin,Rough Toothed Dolphin,Rough-Toothed Dolphins,Sotalia fluviatili,Tucuxi Dolphins,White Beaked Dolphins,White-Beaked Dolphin
D000072317 Polychlorinated Dibenzodioxins Dibenzodioxin derivatives that contain multiple chloride atoms bound to the benzene ring structures. TCDD,Tetrachlorodibenzodioxin,2,3,7,8-Tetrachlorodibenzo-p-dioxin,Chlorinated Dibenzo-p-dioxins,Dibenzo(b,e)(1,4)dioxin, 2,3,7,8-tetrachloro-,PCDD,Polychlorinated Dibenzo-p-dioxins,Polychlorinated Dibenzodioxin,Polychlorodibenzo-4-dioxin,Polychlorodibenzo-p-dioxin,Tetrachlorodibenzo-p-dioxin,Chlorinated Dibenzo p dioxins,Dibenzo-p-dioxins, Chlorinated,Dibenzo-p-dioxins, Polychlorinated,Dibenzodioxin, Polychlorinated,Dibenzodioxins, Polychlorinated,Polychlorinated Dibenzo p dioxins,Polychlorodibenzo 4 dioxin,Polychlorodibenzo p dioxin,Tetrachlorodibenzo p dioxin
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001564 Benzo(a)pyrene A potent mutagen and carcinogen. It is a public health concern because of its possible effects on industrial workers, as an environmental pollutant, an as a component of tobacco smoke. 3,4-Benzopyrene,3,4-Benzpyrene,3,4 Benzopyrene,3,4 Benzpyrene
D001571 Benzoflavones Organic compounds containing a BENZENE ring attached to a flavone group. Some of these are potent arylhydrocarbon hydroxylase inhibitors. They may also inhibit the binding of NUCLEIC ACIDS to BENZOPYRENES and related compounds. The designation includes all isomers; the 7,8-isomer is most frequently encountered. Benzoflavone Compounds,Compounds, Benzoflavone

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