To investigate the effect of growth and maturation on the global kidney clearance of technetium-99m mercaptoacetyltriglycine (99mTc-MAG3), we investigated 509 children who attended for 99mTc-MAG3 renography in our department. In order to estimate the normal maturation of the kidney, only children who were classified as "normal" using diagnostic criteria were included in this study (n = 109). Kidney clearance was calculated using a gamma camera technique and a 20-min blood sample. There was a progressive increase in the clearance of 99mTc-MAG3 throughout childhood and into adolescence (mean clearance value below 2 month was 31.4 ml/min, mean clearance value above 12 years was 287 ml/min). When clearance was normalised to body surface area (BSA) a different pattern was seen, with a progressive increase from 3 weeks of age (mean value < 0.2 years: 208 ml/min/1.73 m2) to a plateau at the end of the first year of life (mean value > 2 years: 303 ml/min/1.73 m2). Maturation of the kidney as defined by the normalised clearance of 99mTc-MAG3 is therefore complete by the end of the 1st year of life, after which any change in non-corrected clearance values may be attributed to growth. To investigate the appropriate normalisation factor for 99mTc-MAG3 clearance in children, clearance values were compared to height, weight and BSA. The relationships were described using a linear model; the correlation coefficients demonstrated that BSA has the highest strength of relationship with 99mTc-MAG3 clearance. Body surface area may be used over 1 year of age to normalise the clearance values of 99mTc-MAG3.(ABSTRACT TRUNCATED AT 250 WORDS)