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Tissue-nonspecific-type alkaline phosphatase is found in the bone, liver, kidney, and other tissues, and its gene consists of 12 exons with the coding sequence beginning in the second exon. Recently, an alternative noncoding first exon was identified in the liver message which differed from that of the previously known osteoblast-derived cDNA sequence. Although these two mRNAs produce an identical protein, they have different promoter regions. The periodontal ligament tissue expresses a high level of alkaline phosphatase activity. To identify its mRNA type, we isolated a full-length cDNA for alkaline phosphatase from a cultured human periodontal ligament cell expression library, using bone-derived tissue-nonspecific alkaline phosphatase cDNA as a hybridization probe. The size of this clone was 2.5 kb, and its 5' and 3' untranslated sequences were identical to those of the human tissue-nonspecific type isolated from osteoblastic cells but not to those of the liver type. In addition, the same fragments as in bone-derived tissue-nonspecific-type cDNA were detected by the treatment of the cDNA clone with restriction enzymes Hinc II and Pst I. The results suggest that expression of the same alkaline phosphatase isozyme in human periodontal ligament cells may be regulated by the same transcriptional mechanism as in bone.
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
November 1988,
Advances in dental research,
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
September 1988,
The Journal of Nihon University School of Dentistry,
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
March 1993,
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology,
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
January 1990,
Nihon Seikeigeka Gakkai zasshi,
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
October 2009,
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology,
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
March 1990,
Kanagawa shigaku. The Journal of the Kanagawa Odontological Society,
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
June 2003,
Journal of periodontology,
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
June 2006,
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology,
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
February 2008,
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology,
M Goseki, and
S Oida, and
K Takeda, and
Y Ogata, and
T Iimura, and
Y Maruoka, and
S Sasaki
October 1994,
General pharmacology,
