Biomaterial Database

Serological typing of red clover necrotic mosaic virus isolates by enzyme-linked immunosorbent assay. 1994

J Gallo, and M Musil

Institute of Virology, Slovak Academy of Sciences, Bratislava.

Enzyme-linked immunosorbent assay (ELISA) enabled a more precise serological typing of isolates of red clover necrotic mosaic virus (RCNMV) originating from Slovak and Czech Republics, Poland, Sweden and Great Britain. We found 5 isolates of serotype A, 14 isolates of serotype B, and 6 isolates of serotype C. Some isolates represented mixtures of serotypes, namely A+B (1 isolate), A+C (1), B+C (7), and A+B+C (5). ELISA was found to be a more suitable method of serotype identification of RCNMV isolates than the double diffusion agar gel test for its higher sensitivity and greater selectivity.

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D009029	Mosaic Viruses	Viruses which produce a mottled appearance of the leaves of plants.	Mosaic Virus,Virus, Mosaic,Viruses, Mosaic
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D000914	Antibodies, Viral	Immunoglobulins produced in response to VIRAL ANTIGENS.	Viral Antibodies
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings
D014773	Virology	The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.