BACKGROUND Beta-endorphin and oxytocin immunocytochemical localization were examined in the neurointermediate lobe (lobus nervosus and pars intermedia) of the bovine hypophysis in order to describe the anatomical distribution of these two neurointermediate lobe hormones. METHODS Twenty-seven bovine hypophyses were collected from slaughterhouse animals (seven mature lactating cows, eleven mature nonlactating cows, three nulliparous heifers, and six steers). Hypophyses were immunostained for oxytocin-containing fibers and beta-endorphin-secreting cells by using the avidin biotin-immunoperoxidase method. The distributions of beta-endorphin-positive cells and oxytocin-positive nerve fibers were plotted on projected outlines of the hypophyses. Immunoreactive staining intensity was graded numerically as weak, moderate, or heavy by three individuals who had no knowledge of the animals' physiological status. RESULTS Oxytocin immunoreactivity was confined to the lobus nervosus while beta-endorphin staining was confined to the pars intermedia and the pars distalis. However, oxytocin immunopositive neurosecretory terminals were distributed more heavily in that part of the lobus nervosus bordering the pars intermedia than in the center of the lobe. CONCLUSIONS These results were similar to those previously reported for the rat (Swaab et al., 1975; J. Neural Transm., 36:195-215; Deftos and Catherwood, 1980; Life Sci., 27:223-228).