Characterization of a murine monoclonal antibody generated against Neospora caninum tachyzoites by use of western blot analysis and immunoelectron microscopy. 1994

R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849.

A murine monoclonal antibody (MAB) 6G7 generated against tachyzoites of Neospora caninum recognized 8 major and several minor antigens, as observed by western blot analysis. Relative rate of migration of the 8 major antigens ranged from 31 to 97.4 kd. In addition, MAB 6G7 recognized a Toxoplasma gondii tachyzoite antigen with a relative rate of migration of 107 kd. Immunogold labeling of N caninum tachyzoites grown in human foreskin fibroblast cells indicated that MAB 6G7 binds to micronemes, dense granules, basal portions of rhoptries, and intravacuolar tubules within the parasitophorous vacuole. Monoclonal antibody 6G7 also bound to micronemes and basal portions of rhoptries within tachyzoites of T gondii. Monoclonal antibody 6G7 did not significantly inhibit development of tachyzoites in vitro.

UI MeSH Term Description Entries
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000913 Antibodies, Protozoan Immunoglobulins produced in a response to PROTOZOAN ANTIGENS. Protozoan Antibodies
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D016253 Microscopy, Immunoelectron Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays. Immunoelectron Microscopy,Microscopy, Immuno-Electron,Immuno-Electron Microscopies,Immuno-Electron Microscopy,Immunoelectron Microscopies,Microscopies, Immuno-Electron,Microscopies, Immunoelectron,Microscopy, Immuno Electron
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D018688 Neospora A genus of protozoan parasites of the subclass COCCIDIA. Its species are parasitic in dogs, cattle, goats, and sheep, among others. N. caninum, a species that mainly infects dogs, is intracellular in neural and other cells of the body, multiplies by endodyogeny, has no parasitophorous vacuole, and has numerous rhoptries. It is known to cause lesions in many tissues, especially the brain and spinal cord as well as abortion in the expectant mother. Neospora caninum,Neospora caninums,Neosporas,caninums, Neospora

Related Publications

R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
January 1992, Parasitology research,
R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
November 2004, The Journal of veterinary medical science,
R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
January 1997, Veterinary parasitology,
R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
April 1987, British journal of cancer,
R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
March 2009, Veterinary parasitology,
R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
June 2004, Journal of veterinary science,
R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
January 2006, The Journal of eukaryotic microbiology,
R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
October 1997, Parasitology,
R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
January 1996, Parasitology research,
R A Cole, and D S Lindsay, and J P Dubey, and M A Toivio-Kinnucan, and B L Blagburn
July 1998, Journal of comparative pathology,
Copied contents to your clipboard!