BIOMAT Database Logo BIOMAT Database Logo

Isoforms of the transmembrane tyrosine phosphatase CD45 differentially affect T cell recognition. 1994

T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510.

Activation of T cells has been shown to require CD45. CD45 is expressed on T cells as distinct isoforms and these isoforms are expressed differentially on subsets of CD4 T cells. We have generated T cell lines expressing a T cell receptor (TCR) of known specificity, with or without CD4, and examined the effect of different CD45 isoforms on stimulation through the antigen receptor. We find that isoforms differ in their ability to participate in antigen recognition, with the null isoform that is predominantly found on memory CD4 T cells being the most effective. The ability of the CD4 T cells being the most effective. The ability of the CD45 ectodomain to differentially affect sensitivity to specific ligands represents a novel way of regulating the efficacy of signaling through a receptor without altering its specificity. It may play a crucial role both in immunological memory and during intrathymic maturation of T cells.

UI MeSH Term Description Entries
D007156 Immunologic Memory The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus. Immune Memory,Immunological Memory,Memory, Immunologic,Immune Memories,Immunologic Memories,Immunological Memories,Memory, Immune,Memory, Immunological
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D011948 Receptors, Antigen, T-Cell Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (CD3 COMPLEX). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains. Antigen Receptors, T-Cell,T-Cell Receptors,Receptors, T-Cell Antigen,T-Cell Antigen Receptor,T-Cell Receptor,Antigen Receptor, T-Cell,Antigen Receptors, T Cell,Receptor, T-Cell,Receptor, T-Cell Antigen,Receptors, T Cell Antigen,Receptors, T-Cell,T Cell Antigen Receptor,T Cell Receptor,T Cell Receptors,T-Cell Antigen Receptors
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
Regulation of integrin-mediated T cell adhesion by the transmembrane protein tyrosine phosphatase CD45.
June 1999, Journal of immunology (Baltimore, Md. : 1950),
T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
Specific CD45 isoforms differentially regulate T cell receptor signaling.
February 1994, The EMBO journal,
T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
New insights into the transmembrane protein tyrosine phosphatase CD45.
November 2001, The international journal of biochemistry & cell biology,
T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
The regulation of B- and T-lymphocyte activation by the transmembrane protein tyrosine phosphatase CD45.
April 1994, Current opinion in cell biology,
T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
Distinct isoforms of the CD45 protein-tyrosine phosphatase differentially regulate interleukin 2 secretion and activation signal pathways involving Vav in T cells.
October 1995, The Journal of biological chemistry,
T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
Correlation between Src family member regulation by the protein-tyrosine-phosphatase CD45 and transmembrane signaling through the T-cell receptor.
February 1993, Proceedings of the National Academy of Sciences of the United States of America,
T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
Determination of CD45 tyrosine phosphatase activity in T lymphocytes.
January 2000, Methods in molecular biology (Clifton, N.J.),
T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
CD45: a transmembrane protein tyrosine phosphatase involved in the transduction of antigenic signals.
February 1992, Biochemical Society transactions,
T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
The transmembrane protein-tyrosine phosphatase CD45 is associated with decreased insulin receptor signaling.
January 1996, The Journal of biological chemistry,
T J Novak, and D Farber, and D Leitenberg, and S C Hong, and P Johnson, and K Bottomly
Mutation of the receptor tyrosine phosphatase PTPRC (CD45) in T-cell acute lymphoblastic leukemia.
May 2012, Blood,
Copied contents to your clipboard!