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In this study, we demonstrate the role of M cells in uptake of poly(D-L-lactic-co-glycolic acid) (PLGA) microspheres and transport into rabbit Peyer's patches. Microspheres 1 to 10 microns in diameter composed of 50:50 lactic acid:glycolic acid were instilled into intestinal segments containing jejunal or ileal Peyer's patches, and uptake by M cells was examined by electron microscopy. PLGA microspheres visualized as electron-lucent, spherical particles were taken up by M cells by pseudopod-like extensions of the M cell apical membrane and translocated to the pocket region containing mononuclear leukocytes within 60 min. These results indicate that PLGA microspheres can be directed to M cell apical surfaces for delivery to immunocompetent cells in gut-associated lymphoid tissues.
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
March 1987,
Cell and tissue research,
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
August 1986,
Gastroenterologia Japonica,
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
July 1991,
Immunology,
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
November 1992,
Experimental physiology,
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
August 1997,
Science (New York, N.Y.),
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
April 1989,
Clinical and experimental immunology,
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
January 2004,
The American journal of pathology,
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
March 1998,
Vaccine,
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
March 2004,
Journal of immunology (Baltimore, Md. : 1950),
T H Ermak, and
E P Dougherty, and
H R Bhagat, and
Z Kabok, and
J Pappo
May 2000,
International journal of pharmaceutics,
