-transparent.png){kind=logo}
The baculovirus expression system was used to produce three different constructs of the murine cell surface adhesion receptor CD2. One construct coded for a single, N-terminal, Ig-fold domain. It was inefficiently secreted and therefore primarily intracellular. The second construct coded for both extracellular, N-terminal Ig-fold domains. This was efficiently secreted into culture supernatant. The third construct coded for the full-length transmembrane molecule which localized to the cell surface. All constructs were monomers of predicted MWr and were appropriately glycosylated. They retained epitopic specificity as demonstrated by binding to mAbs, and adhesion function as demonstrated by a rosetting assay.
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
January 2019,
Methods in molecular biology (Clifton, N.J.),
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
August 1987,
Journal of immunological methods,
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
January 1987,
Journal of lipid research,
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
November 1998,
Human gene therapy,
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
January 1990,
Advances in experimental medicine and biology,
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
December 1989,
Virology,
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
January 2023,
Methods in molecular biology (Clifton, N.J.),
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
July 2021,
Journal of visualized experiments : JoVE,
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
January 2021,
Methods in molecular biology (Clifton, N.J.),
A J Kaplan, and
K D Chavin, and
L H Qin, and
H Yagita, and
J S Bromberg
January 2016,
Methods in molecular biology (Clifton, N.J.),
