Biomaterial Database

Demonstration of human nephritogenic tubular antigen in the serum and organs by radioimmunoassay. 1976

Y Miyakawa, and K Kitamura, and S Shibata, and Naruse

Nephritogenic tubular epithelial antigen (Tub-Ag), which had been found in immune complexes deposited along the glomerular capillary walls of some patients with idiopathic membranous glomerulonephritis, was solubilized from renal tubuli of humans by Pronase digestion. Soluble Tub-Ag was then purified by gel filtration and ion exchange chromatography on DEAE-Sephadex. Three DEAE fractions, designated as 0.07 M, 0.13M, and 0.23 M fractions, raised antibodies in rabbits and fluoresceinated antibodies against any of these fractions reacted exclusively with luminal layer of proximal tubular epithelia. Among three fluoresceinated antibodies, however, only the one directed to 0.07 M fraction bound with immune complexes which were deposited along the glomerular capilary walls of the patients with Tub-Ag (DEAE 0.07 M fraction) was physicochemically homogenous with a S20 value of 8.2. Utilizing 125I-labeled Tub-Ag, a sensitive and quantitave radioimmunoassay of Tub-Ag was developed. Tub-Ag was demonstrated to occur naturally in serum and urine, as well as in all the organs tested including kidney, intestine, liver, spleen, stomach, heart, and lung. Tub-Ag was detected even in the sera of anephric patients on maintenance hemodialysis (60.8 +/- 7.8 radioimmunoassay units/ml), although at slightly lower levels than in those of normal individuals (69.9 +/- 10.6 units/ml). The size of serum Tub-Ag was identical to that of molecules bearing Tub-Ag activity solubilized by Pronase from all the organs tested, indicating that circulating Tub-Ag was maintained mainly by organs other than kidneys.

UI	MeSH Term	Description	Entries
D007422	Intestines	The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.	Intestine
D007672	Kidney Cortex	The outer zone of the KIDNEY, beneath the capsule, consisting of KIDNEY GLOMERULUS; KIDNEY TUBULES, DISTAL; and KIDNEY TUBULES, PROXIMAL.	Cortex, Kidney
D007684	Kidney Tubules	Long convoluted tubules in the nephrons. They collect filtrate from blood passing through the KIDNEY GLOMERULUS and process this filtrate into URINE. Each renal tubule consists of a BOWMAN CAPSULE; PROXIMAL KIDNEY TUBULE; LOOP OF HENLE; DISTAL KIDNEY TUBULE; and KIDNEY COLLECTING DUCT leading to the central cavity of the kidney (KIDNEY PELVIS) that connects to the URETER.	Kidney Tubule,Tubule, Kidney,Tubules, Kidney
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008168	Lung	Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.	Lungs
D009206	Myocardium	The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.	Muscle, Cardiac,Muscle, Heart,Cardiac Muscle,Myocardia,Cardiac Muscles,Heart Muscle,Heart Muscles,Muscles, Cardiac,Muscles, Heart
D011402	Pronase	A proteolytic enzyme obtained from Streptomyces griseus.	Pronase E,Pronase P,Protease XIV,XIV, Protease
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D005455	Fluorescent Antibody Technique	Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.	Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man