Biomaterial Database

Morphology of in vitro sperm phagocytosis by rat peritoneal macrophages under influence of oily contrast medium (Lipiodol). 1994

D Mikulska, and R Kurzawa, and L Ròzewicka

Department of Histology and Embryology, Pomeranian Medical Academy, Szczecin, Poland.

Morphology of sperm phagocytosing rat peritoneal macrophages (PM) in control and Lipiodol added cultures was evaluated under light as well as scanning electron and transmission electron microscopes. The spermiophagic index (SPI) was assessed to estimate an inhibitory effect of Lipiodol on sperm phagocytosis to be compared with the outcome of morphological studies. Lipiodol inhibits in vitro sperm phagocytosis as indicated by SPI and evaluation under light, scanning electron and transmission electron microscopes. The highest SPI was seen in control cultures (14.44), declined in cultures with 0.25% and 0.5% Lipiodol (3.3 and 3.02 respectively) to reach the lowest value with 1% Lipiodol (1.60). The macrophages in cultures with Lipiodol were covered by a thin layer of oily contrast medium. The layer produced changes in the macrophages' shape and the cytoplasmic surface configuration. Lipiodol hinders both sperm phagocytosis and movement of PM by altering their shape and impairing pseudopodia and protrusions formation. Morphological studies of sperm phagocytosis by PM can help explain therapeutic effects of oily contrast media used in hysterosalpingography. Number of studies have suggested therapeutic effects of hysterosalpingography (HSG). These studies have demonstrated an increase in pregnancy rates after HSG in previously infertile women (Fullenlove, 1969; Palmer, 1960). Pregnancies were even more numerous after applying oil, than water based contrast media (DeCharney et al., 1980; Schwabe, 1983). Sawatari et al., 1993 have lately shown, that Lipiodol significantly increases pregnancy rates in animals. Hypothesis has been put forward as to the possibility of modulating activity of these agents exerted upon the peritoneal and oviductal macrophages (Boyer et al., 1986; Schwabe et al., 1983).(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D007459	Iodized Oil	A preparation of oil that contains covalently bound IODINE. It is commonly used as a RADIOCONTRAST AGENT and as a suspension medium for CHEMOTHERAPEUTIC AGENTS.	Oil, Iodized,Iodized Oils,Iodolipol,Oils, Iodized
D008297	Male		Males
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D008855	Microscopy, Electron, Scanning	Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.	Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013094	Spermatozoa	Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.	Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms
D017208	Rats, Wistar	A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.	Wistar Rat,Rat, Wistar,Wistar Rats
D017737	Macrophages, Peritoneal	Mononuclear phagocytes derived from bone marrow precursors but resident in the peritoneum.	Peritoneal Macrophages,Macrophage, Peritoneal,Peritoneal Macrophage