-transparent.png){kind=logo}
5-Lipoxygenase (5-LO) converts arachidonic acid, released from membrane phospholipids upon external stimulation, to leukotriene C4 (LTC4), which induces various kinds of cellular and molecular responses. We examined the effects of 5 min of ischemia on brain 5-LO and LTC4 during reperfusion using the gerbil model of transient forebrain ischemia that develops neuronal necrosis selectively in the hippocampus. Neurons exhibited dense 5-LO immunoreactivity; 5-LO was partially redistributed from cytosolic to particulate fractions 3 min during reperfusion. LTC4 was generated in neurons and was increased in all forebrain regions during reperfusion. Postischemic increases in LTC4 were inhomogeneous; a greater increase was observed in the hippocampus (13.37 +/- 0.24 pmol/g tissue) than in the other regions (cerebral cortex: 3.29 +/- 1.09 pmol/g). Superoxide dismutase and dimethylthiourea, oxygen radical scavengers, attenuated the production of LTC4 and damage to the neurons in the hippocampus during reperfusion. Our findings indicated that reperfusion, which was associated with translocation of cytosolic 5-LO to membranes and generation of oxygen radicals, induced the production of LTC4 and suggested that excess LTC4 production may mediate irreversible reperfusion injuries in the hippocampal neurons.
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
January 1997,
Canadian journal of physiology and pharmacology,
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
April 1997,
The American journal of physiology,
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
April 1992,
Cell,
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
December 1983,
Proceedings of the National Academy of Sciences of the United States of America,
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
January 1994,
Advances in prostaglandin, thromboxane, and leukotriene research,
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
October 1995,
Journal of medicinal chemistry,
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
January 2008,
The Journal of experimental medicine,
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
October 2004,
Journal of enzyme inhibition and medicinal chemistry,
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
February 1989,
Stroke,
T Ohtsuki, and
M Matsumoto, and
Y Hayashi, and
K Yamamoto, and
K Kitagawa, and
S Ogawa, and
S Yamamoto, and
T Kamada
April 2003,
The Journal of asthma : official journal of the Association for the Care of Asthma,
