Electrophysiological properties and chemosensitivity of acutely isolated nodose ganglion neurons of the rabbit. 1993

H Leal-Cardoso, and G M Koschorke, and G Taylor, and D Weinreich
University of Maryland School of Medicine, Department of Pharmacology and Experimental Therapeutics, Baltimore 21201-1559.

Neurons in the nodose ganglion were enzymatically dissociated from adult rabbits and electrophysiologically examined within 2 h. About 90% of the neurons survived the dissociation procedure. Their resting membrane potential (-59 +/- 0.6 mV), time constant (5.7 +/- 0.5 ms), input resistance (80 +/- 5.3 M omega), action potential properties (amplitude, 79 +/- 1.2 mV; overshoot 21 +/- 1.0 mV; duration 5.2 +/- 0.1 ms; threshold, -38 +/- 0.9 mV; and fast spike afterhyperpolarization, 10 +/- 0.5 mV, 64 +/- 3.8 ms) were not different from values obtained from nodose neurons in intact ganglion in vitro and in vivo. An unusually long duration spike afterhyperpolarization observed in about 35% of nodose neurons in intact ganglion was demonstrated in about 28% of the acutely isolated neurons. The percentage of isolated neurons responding to bath applied acetylcholine, bradykinin, serotonin and histamine was similar to those reported for neurons in the intact ganglia. We conclude that the electrophysiological and chemoreceptive properties of acutely isolated adult rabbit nodose neurons are similar to those observed for neurons in intact ganglia. Thus the availability of large numbers of acutely isolated adult nodose neurons should allow multidisciplinary studies of vagal afferent C-fiber neurons not readily obtainable in the intact ganglion.

UI MeSH Term Description Entries
D008566 Membranes Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures. Membrane Tissue,Membrane,Membrane Tissues,Tissue, Membrane,Tissues, Membrane
D008839 Microelectrodes Electrodes with an extremely small tip, used in a voltage clamp or other apparatus to stimulate or record bioelectric potentials of single cells intracellularly or extracellularly. (Dorland, 28th ed) Electrodes, Miniaturized,Electrode, Miniaturized,Microelectrode,Miniaturized Electrode,Miniaturized Electrodes
D009412 Nerve Fibers Slender processes of NEURONS, including the AXONS and their glial envelopes (MYELIN SHEATH). Nerve fibers conduct nerve impulses to and from the CENTRAL NERVOUS SYSTEM. Cerebellar Mossy Fibers,Mossy Fibers, Cerebellar,Cerebellar Mossy Fiber,Mossy Fiber, Cerebellar,Nerve Fiber
D009474 Neurons The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM. Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D009620 Nodose Ganglion The inferior (caudal) ganglion of the vagus (10th cranial) nerve. The unipolar nodose ganglion cells are sensory cells with central projections to the medulla and peripheral processes traveling in various branches of the vagus nerve. Nodose Ganglia,Ganglia, Nodose,Ganglion, Nodose
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002628 Chemoreceptor Cells Cells specialized to detect chemical substances and relay that information centrally in the nervous system. Chemoreceptor cells may monitor external stimuli, as in TASTE and OLFACTION, or internal stimuli, such as the concentrations of OXYGEN and CARBON DIOXIDE in the blood. Chemoreceptive Cells,Cell, Chemoreceptive,Cell, Chemoreceptor,Cells, Chemoreceptive,Cells, Chemoreceptor,Chemoreceptive Cell,Chemoreceptor Cell
D004594 Electrophysiology The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D000200 Action Potentials Abrupt changes in the membrane potential that sweep along the CELL MEMBRANE of excitable cells in response to excitation stimuli. Spike Potentials,Nerve Impulses,Action Potential,Impulse, Nerve,Impulses, Nerve,Nerve Impulse,Potential, Action,Potential, Spike,Potentials, Action,Potentials, Spike,Spike Potential
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

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