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The effect of mitomycin C (MMC) treatment on gene amplification and gene deletion induction in a V79/AP4 Chinese hamster cell line was investigated. Spontaneous and induced cellular variants resistant to N-phosphonacetyl-L-aspartate (PALA), which selects for carbamyl-P-synthetase, aspartate transcarbamylase and dihydroorotase (CAD) gene amplification events, and to intermediate concentrations of 2,6-diaminopurine (DAP), which selects for adenine phosphoribosyl transferase (aprt) gene deletion events, were isolated. The molecular analysis of spontaneous and induced PALA- and DAP-resistant clones showed that while in the former the CAD gene was amplified, in the latter both mutations and deletions occurred at the aprt gene. These results indicate that MMC favored the rise of gene amplification rather than gene deletion events, and suggest that aberrant DNA replication processes were responsible for the observed gene amplification.
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
November 1988,
Somatic cell and molecular genetics,
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
January 1988,
Chromosoma,
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
June 1984,
Journal of cellular physiology,
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
January 1989,
Mutation research,
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
November 1988,
Somatic cell and molecular genetics,
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
June 1989,
Genetical research,
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
October 1982,
Chemico-biological interactions,
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
November 1998,
Mutation research,
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
November 1996,
Biochemical and biophysical research communications,
L Vatteroni, and
A Piras, and
A Moretti, and
G Rainaldi
December 1977,
Chemico-biological interactions,
