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Some vegetable extract have haemagglutinating activity and are able to transform lymphocytes in culture. The comparative activities of lectin from Pisum sativum and other lectins already used (phytohaere shown. This lectin can be isolated in a highly purified form which is described. Purification of the human blood lymphocytes is obtained through nylon columns and measurement of transformation and proliferation by means of 3 H thymidine incorporation. Normal lymphocyte stimulation can be obtained within a range of concentrations of stimulants can be obtained within a range of concentrations of stimulants and the curves with PHA or lectin from Pisum show a peak of blastogenic activity. The activity of lectin from Pisum sativum was studied in this way on blood cultures of 8 chronic lymphocytic leukaemias and 5 immunodeficient patients. It appeared that lectin from Pisum and PHA have the same activity. The response of human peripheral lymphocytes to various mitogenic agents in vitro does not point out the proliferative abnormal cell and may not be used to detect different populations of human lymphocytes.
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
January 1969,
Comptes rendus des seances de la Societe de biologie et de ses filiales,
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
July 1995,
Indian journal of pathology & microbiology,
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
October 1994,
Journal of immunological methods,
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
February 1975,
Biochimica et biophysica acta,
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
September 1987,
Nucleic acids research,
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
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Biological chemistry Hoppe-Seyler,
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
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The Journal of biological chemistry,
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
September 1980,
[Hokkaido igaku zasshi] The Hokkaido journal of medical science,
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
June 1973,
Revista espanola de fisiologia,
I Bernard-Griffiths, and
G Betail, and
D Godeneche, and
M Coulet, and
C Herzog, and
J L Binet
March 1988,
Planta,
