Biomaterial Database

A mutant of Saccharomyces cerevisiae defective for nuclear fusion. 1976

J Conde, and G R Fink

A mutant unable to fuse nuclei during mating has been isolated from standard wild-type Saccharomyces cerevisiae. Tetrad analysis of the mutation responsible for this defect (kar1-1) shows that it segregates as a single Mendelian factor. The defect kn kaf1-1 appears to be nuclear limited. Cytological and genetic evidence shows that in this mutant the events associated with zygote formation are normal until the point of nuclear fusion. The consequence of this defect is the formation of a multinucleate zygote which in subsequent divisions can segregate heterokaryons and haploid heterplasmons.

UI	MeSH Term	Description	Entries
D008540	Meiosis	A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.	M Phase, Meiotic,Meiotic M Phase,M Phases, Meiotic,Meioses,Meiotic M Phases,Phase, Meiotic M,Phases, Meiotic M
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D012098	Reproduction	The total process by which organisms produce offspring. (Stedman, 25th ed)	Human Reproductive Index,Human Reproductive Indexes,Reproductive Period,Human Reproductive Indices,Index, Human Reproductive,Indexes, Human Reproductive,Indices, Human Reproductive,Period, Reproductive,Periods, Reproductive,Reproductive Index, Human,Reproductive Indices, Human,Reproductive Periods
D002459	Cell Fusion	Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.	Cell Fusions,Fusion, Cell,Fusions, Cell
D002467	Cell Nucleus	Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D005260	Female		Females
D006238	Haploidy	The chromosomal constitution of cells, in which each type of CHROMOSOME is represented once. Symbol: N.	Haploid,Haploid Cell,Cell, Haploid,Cells, Haploid,Haploid Cells,Haploidies,Haploids
D012441	Saccharomyces cerevisiae	A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.	Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D015053	Zygote	The fertilized OVUM resulting from the fusion of a male and a female gamete.	Fertilized Ovum,Ovum, Fertilized,Fertilized Egg,Egg, Fertilized,Eggs, Fertilized,Fertilized Eggs,Zygotes