Biomaterial Database

[Polymorphism of Helicobacter pylori urease genes and gastric diseases]. 1993

T Sugiyama, and T Awakawa

Department of Internal Medicine (Section 1), Sapporo Medical University School of Medicine.

Helicobacter pylori (HP) is now recognized as a major etiologic agent of chronic gastritis and peptic ulcer and is recently presumed to be a cofactor in the occurrence of gastric cancer. HP has a high urease activity, which possibly plays a role in the pathogenesis of gastroduodenal diseases. In this paper, the function and the genetic structure of HP urease gene and the restriction fragment length polymorphism (RFLP) among HP strains using a probe of HP urease alpha subunit gene were described. HP urease gene cluster, which included structure genes, regulatory genes and accessory genes was identified and associated with the activity and the assembly. The RFLP pattern of HP tends to be distinct in gastric diseases, 460 bp pattern in gastric ulcer and 1,100 bp pattern in chronic gastritis, by Hae III digestion. HP showing a 460 bp RFLP pattern possessed a relatively high urease activity.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D012150	Polymorphism, Restriction Fragment Length	Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.	RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D013272	Stomach Diseases	Pathological processes involving the STOMACH.	Gastric Diseases,Disease, Gastric,Disease, Stomach,Diseases, Gastric,Diseases, Stomach,Gastric Disease,Stomach Disease
D014510	Urease	An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5.	Phytourease,Urea Amidohydrolase,Amidohydrolase, Urea
D016480	Helicobacter pylori	A spiral bacterium active as a human gastric pathogen. It is a gram-negative, urease-positive, curved or slightly spiral organism initially isolated in 1982 from patients with lesions of gastritis or peptic ulcers in Western Australia. Helicobacter pylori was originally classified in the genus CAMPYLOBACTER, but RNA sequencing, cellular fatty acid profiles, growth patterns, and other taxonomic characteristics indicate that the micro-organism should be included in the genus HELICOBACTER. It has been officially transferred to Helicobacter gen. nov. (see Int J Syst Bacteriol 1989 Oct;39(4):297-405).	Campylobacter pylori,Campylobacter pylori subsp. pylori,Campylobacter pyloridis,Helicobacter nemestrinae